Anti-ACSL4 Antibody (6H118) is a Rabbit antibody targeting ACSL4. Anti-ACSL4 Antibody (6H118) can be used in IF,IHC-Fr,IHC-P,IP,WB.

IgG

6H118

Human,Mouse,Rat

1. 25 μg total protein per Lane of various lysates probed with ACSL4 monoclonal antibody, unconjugated (TMAB-00043) at 1:2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min.
2. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation ACSL4 Monoclonal Antibody, Unconjugated (TMAB-00043) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation ACSL4 Monoclonal Antibody, Unconjugated (TMAB-00043) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation ACSL4 Monoclonal Antibody, Unconjugated (TMAB-00043) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Mouse Duodenum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation ACSL4 Monoclonal Antibody, Unconjugated (TMAB-00043) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded Human Duodenum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation ACSL4 Monoclonal Antibody, Unconjugated (TMAB-00043) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation ACSL4 Monoclonal Antibody, Unconjugated (TMAB-00043) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
8. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation ACSL4 Monoclonal Antibody, Unconjugated (TMAB-00043) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
9. Paraformaldehyde-fixed, paraffin embedded Rat Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation ACSL4 Monoclonal Antibody, Unconjugated (TMAB-00043) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
10. Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation ACSL4 Monoclonal Antibody, Unconjugated (TMAB-00043) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; IP: 1:20-50

Monoclonal

A synthesized peptide: human ACSL4

Human

ACSL4

Long-chain-fatty-acid--CoA ligase 4

Lipid metabolism,Lipid Signaling

Activation of long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. Preferentially uses arachidonate and eicosapentaenoate as substrates.