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Anti-ACSL4 Antibody (6H118) is a Rabbit antibody targeting ACSL4. Anti-ACSL4 Antibody (6H118) can be used in WB,IHC-P,IHC-Fr,IF,IP.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
25 μL | $153 | 7-10 days | |
50 μL | $272 | 7-10 days | |
100 μL | $487 | 7-10 days |
Description | Antibody Type: Recombinant Rabbit Monoclonal Application: WB,IHC-P,IHC-Fr,IF,IP Reactivity: Human,Mouse,Rat |
Ig Type | IgG |
Clone | 6H118 |
Reactivity | Human,Mouse,Rat |
Verified Activity | 1. 25 μg total protein per Lane of various lysates probed with ACSL4 monoclonal antibody, unconjugated (TMAB-00043) at 1:2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min. 2. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation ACSL4 Monoclonal Antibody, Unconjugated (TMAB-00043) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation ACSL4 Monoclonal Antibody, Unconjugated (TMAB-00043) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation ACSL4 Monoclonal Antibody, Unconjugated (TMAB-00043) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 5. Paraformaldehyde-fixed, paraffin embedded Mouse Duodenum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation ACSL4 Monoclonal Antibody, Unconjugated (TMAB-00043) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 6. Paraformaldehyde-fixed, paraffin embedded Human Duodenum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation ACSL4 Monoclonal Antibody, Unconjugated (TMAB-00043) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 7. Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation ACSL4 Monoclonal Antibody, Unconjugated (TMAB-00043) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 8. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation ACSL4 Monoclonal Antibody, Unconjugated (TMAB-00043) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 9. Paraformaldehyde-fixed, paraffin embedded Rat Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation ACSL4 Monoclonal Antibody, Unconjugated (TMAB-00043) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 10. Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation ACSL4 Monoclonal Antibody, Unconjugated (TMAB-00043) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. |
Application | WB,IHC-P,IHC-Fr,IF,IP |
Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; IP: 1:20-50 |
Antibody Type | Monoclonal |
Host Species | Rabbit |
Subcellular Localization | Mitochondrion outer membrane. Peroxisome membrane. Microsome membrane. Endoplasmic reticulum membrane. |
Construction | Recombinant Antibody |
Purification | Protein A purified |
Appearance | Liquid |
Formulation | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol. |
Research Background | Catalyzes the conversion of long-chain fatty acids to their active form acyl-CoA for both synthesis of cellular lipids, and degradation via beta-oxidation. Preferentially activates arachidonate and eicosapentaenoate as substrates. |
Immunogen | A synthesized peptide: human ACSL4 |
Antigen Species | Human |
Gene ID | 2182 |
Uniprot ID | |
Biology Area | Lipid metabolism,Lipid Signaling |
Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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