Anti-ACP5 Polyclonal Antibody 2 is a Rabbit antibody targeting ACP5. Anti-ACP5 Polyclonal Antibody 2 can be used in FCM.

IgG

Human (predicted:Mouse,Rat,Pig)

Blank control (black line): Hela.
Primary Antibody (green line): Rabbit Anti-TRAP5 antibody (TMAB-00040)
Dilution: 2 μg/Test;
Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488
Dilution: 0.5 μg/Test.
Isotype control (orange line): Normal Rabbit IgG
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20°C, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.

FCM: 2ug/Test

Polyclonal

KLH conjugated synthetic peptide: human TRAP5

Human

ACP5

Tartrate-resistant acid phosphatase type 5

Phosphatases,Phosphatases,Other Metabolism Assay

Defects in ACP5 are the cause of spondyloenchondrodysplasia with immune dysregulation (SPENCDI) [MIM:607944]. A disease characterized by vertebral and metaphyseal dysplasia, spasticity with cerebral calcifications, and strong predisposition to autoimmune diseases. The skeletal dysplasia is characterized by radiolucent and irregular spondylar and metaphyseal lesions that represent islands of chondroid tissue within bone. Note=ACP5 inactivating mutations result in a functional excess of phosphorylated osteopontin causing deregulation of osteopontin signaling and consequential autoimmune disease.