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Anti-ACP5 Polyclonal Antibody

😃Good
Catalog No. TMAB-00039

Anti-ACP5 Polyclonal Antibody is a Rabbit antibody targeting ACP5. Anti-ACP5 Polyclonal Antibody can be used in WB,FCM.

Anti-ACP5 Polyclonal Antibody

Anti-ACP5 Polyclonal Antibody

😃Good
Catalog No. TMAB-00039
Anti-ACP5 Polyclonal Antibody is a Rabbit antibody targeting ACP5. Anti-ACP5 Polyclonal Antibody can be used in WB,FCM.
Pack SizePriceAvailabilityQuantity
50 μL$220 7-10 days
100 μL$372 7-10 days
200 μL$527 7-10 days
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Product Introduction

Bioactivity
Description
Anti-ACP5 Polyclonal Antibody is a Rabbit antibody targeting ACP5. Anti-ACP5 Polyclonal Antibody can be used in WB,FCM.
Ig Type
IgG
Reactivity
Human,Mouse (predicted:Rat,Dog,Pig,Horse,Rabbit)
Verified Activity
1. Sample:
Liyer (Mouse) Cell Lysate at 40 μg
Primary: Anti-Tartrate Resistant Acid Phosphatase (TMAB-00039) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 34 kDa
Observed band size: 34 kDa
2. Sample:
MOLT-4 (Human) Cell Lysate at 30 μg
Primary: Anti-Tartrate Resistant Acid Phosphatase (TMAB-00039) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 34 kDa
Observed band size: 35 kDa
3. Blank control (black line): Hela.
Primary Antibody (green line): Rabbit Anti-ACP5 antibody (TMAB-00039)
Dilution: 1 μg/Test;
Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488
Dilution: 0.5 μg/Test.
Isotype control (orange line): Normal Rabbit IgG
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20°C, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
Application
WB,FCM
Recommended Dose
WB: 1:500-2000; FCM: 1ug/Test
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationLysosome.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundThis gene encodes an iron containing glycoprotein which catalyzes the conversion of orthophosphoric monoester to alcohol and orthophosphate. It is the most basic of the acid phosphatases and is the only form not inhibited by L(+)-tartrate. [provided by RefSeq, Aug 2008]
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human TRAP/Tartrate Resistant Acid Phosphatase
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Phosphatases,Phosphatases,Other Metabolism Assay
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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