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Anti-ACP5 Polyclonal Antibody is a Rabbit antibody targeting ACP5. Anti-ACP5 Polyclonal Antibody can be used in WB,FCM.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $220 | 7-10 days | |
100 μL | $372 | 7-10 days | |
200 μL | $527 | 7-10 days |
Description | Anti-ACP5 Polyclonal Antibody is a Rabbit antibody targeting ACP5. Anti-ACP5 Polyclonal Antibody can be used in WB,FCM. |
Ig Type | IgG |
Reactivity | Human,Mouse (predicted:Rat,Dog,Pig,Horse,Rabbit) |
Verified Activity | 1. Sample: Liyer (Mouse) Cell Lysate at 40 μg Primary: Anti-Tartrate Resistant Acid Phosphatase (TMAB-00039) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 34 kDa Observed band size: 34 kDa 2. Sample: MOLT-4 (Human) Cell Lysate at 30 μg Primary: Anti-Tartrate Resistant Acid Phosphatase (TMAB-00039) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 34 kDa Observed band size: 35 kDa 3. Blank control (black line): Hela. Primary Antibody (green line): Rabbit Anti-ACP5 antibody (TMAB-00039) Dilution: 1 μg/Test; Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488 Dilution: 0.5 μg/Test. Isotype control (orange line): Normal Rabbit IgG Protocol The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20°C, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. |
Application | WB,FCM |
Recommended Dose | WB: 1:500-2000; FCM: 1ug/Test |
Antibody Type | Polyclonal |
Host Species | Rabbit |
Subcellular Localization | Lysosome. |
Construction | Hybridoma Polyclonal Antibody |
Purification | Protein A purified |
Appearance | Liquid |
Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
Research Background | This gene encodes an iron containing glycoprotein which catalyzes the conversion of orthophosphoric monoester to alcohol and orthophosphate. It is the most basic of the acid phosphatases and is the only form not inhibited by L(+)-tartrate. [provided by RefSeq, Aug 2008] |
Immunogen | KLH conjugated synthetic peptide: human TRAP/Tartrate Resistant Acid Phosphatase |
Antigen Species | Human |
Gene ID | |
Uniprot ID | |
Biology Area | Phosphatases,Phosphatases,Other Metabolism Assay |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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