Anti-ACP5 Polyclonal Antibody is a Rabbit antibody targeting ACP5. Anti-ACP5 Polyclonal Antibody can be used in WB,FCM.

IgG

Human,Mouse (predicted:Rat,Dog,Pig,Horse,Rabbit)

1. Sample:
Liyer (Mouse) Cell Lysate at 40 μg
Primary: Anti-Tartrate Resistant Acid Phosphatase (TMAB-00039) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 34 kDa
Observed band size: 34 kDa
2. Sample:
MOLT-4 (Human) Cell Lysate at 30 μg
Primary: Anti-Tartrate Resistant Acid Phosphatase (TMAB-00039) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 34 kDa
Observed band size: 35 kDa
3. Blank control (black line): Hela.
Primary Antibody (green line): Rabbit Anti-ACP5 antibody (TMAB-00039)
Dilution: 1 μg/Test;
Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488
Dilution: 0.5 μg/Test.
Isotype control (orange line): Normal Rabbit IgG
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20°C, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.

WB: 1:500-2000; FCM: 1ug/Test

Polyclonal

KLH conjugated synthetic peptide: human TRAP/Tartrate Resistant Acid Phosphatase

Human

Phosphatases,Phosphatases,Other Metabolism Assay