Anti-Acetyl Lysine Antibody (7U108) is a Rabbit antibody targeting Acetyl Lysine. Anti-Acetyl Lysine Antibody (7U108) can be used in WB,IHC-P,IHC-Fr,IF.

IgG

7U108

Species independent

1. Sealing solution and concentration: 5% NFDM/TBST
Cracking liquid: HeLa
First antibody dilution: 1:1000
Incubation conditions for primary antibody: 2 hours at room temperature
Secondary antibody: Goat Anti Rabbit IgG H&L (HRP)
Processing method: 1: PTM-105RM pre adorbed with 3 μ M synthetic Kac peptides; 2: PTM-105RM pre adorbed with 3 μ M unmodified peptides
Protein loading amount: 20 μg
Exposure time: 30 seconds
Theoretical molecular weight: Multiple kDa
Actual molecular weight: Multiple kDa
2. Sealing solution and concentration: 5% NFDM/TBST
First antibody dilution: 1:1000
Incubation conditions for primary antibody: 2 hours at room temperature
Cracking solution: 1: HeLa, 2: NIH-3T3, 3: BRL, 4: Mouse liver, 5: Mouse kidney
Secondary antibody: Goat Anti Rabbit IgG H&L (HRP)
Protein loading amount: 20 μg
Exposure time: 60 seconds
Theoretical molecular weight: Multiple kDa
Actual molecular weight: Multiple kDa
3. Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Acetyl Lysine Monoclonal Antibody, Unconjugated (TMAB-02205) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Acetyl Lysine Monoclonal Antibody, Unconjugated (TMAB-02205) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.

WB: 1:500-1000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Monoclonal