Anti-Acetyl Lysine Antibody (2S102) is a Mouse antibody targeting Acetyl Lysine. Anti-Acetyl Lysine Antibody (2S102) can be used in IHC-P,IHC-Fr,ICC/IF,IF,FCM.

IgG

2S102

Species independent

1. The Hela (Treated Sodium Butyrate (5 mM, 24 h)) (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.). Primary Antibody (green): Mouse Anti-Acetyl Lysine antibody (TMAB-02204): 1 μg/10^6 cells; Secondary Antibody (white blue): Goat anti-Mouse IgG-BF488: 1 μg/test. Blank control (black): PBS. Acquisition of 20,000 events was performed.
2. 4% Paraformaldehyde-fixed Hela (Treated Sodium Butyrate (5 mM, 24 h)） (H) cell; Triton X-100 at r. T. for 20 min; Antibody incubation with (Acetyl Lysine) monoclonal Antibody, unconjugated (TMAB-02204) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Mouse IgG antibody (green) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:50-200; IF: 1:100-500; FCM: 1μg/Test

Monoclonal

BSA conjugated Acetylated Lysine