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Anti-Acetyl-Histone H4 (Lys5) Polyclonal Antibody 3
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Catalog No. TMAB-07136
Anti-Acetyl-Histone H4 (Lys5) Polyclonal Antibody 3 is a Rabbit antibody targeting Acetyl-Histone H4 (Lys5). Anti-Acetyl-Histone H4 (Lys5) Polyclonal Antibody 3 can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.
Anti-Acetyl-Histone H4 (Lys5) Polyclonal Antibody 3
Anti-Acetyl-Histone H4 (Lys5) Polyclonal Antibody 3
Copy Product Info😃Good
Catalog No. TMAB-07136
Anti-Acetyl-Histone H4 (Lys5) Polyclonal Antibody 3 is a Rabbit antibody targeting Acetyl-Histone H4 (Lys5). Anti-Acetyl-Histone H4 (Lys5) Polyclonal Antibody 3 can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $220 | 7-10 days | 7-10 days | |
| 100 μL | $373 | 7-10 days | 7-10 days | |
| 200 μL | $527 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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| Description | Anti-Acetyl-Histone H4 (Lys5) Polyclonal Antibody 3 is a Rabbit antibody targeting Acetyl-Histone H4 (Lys5). Anti-Acetyl-Histone H4 (Lys5) Polyclonal Antibody 3 can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF. |
| Ig Type | IgG |
| Reactivity | Human,Rat (predicted:Mouse,Dog,Pig,Cow,Horse,Rabbit) |
| Verified Activity | 1. Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (Histone H4 (Acetyl K5)) polyclonal Antibody, Unconjugated (TMAB-07136) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei. 2. Sample: Lane 1: Human Hela cell lysates Lane 2: Human Jurkat cell lysates Lane 3: Human HL60 cell lysates Lane 4: Human MCF-7 cell lysates Primary: Anti-Histone H4 (Acetyl K5) (TMAB-07136) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 11 kD Observed band size: 11 kD 3. Paraformaldehyde-fixed, paraffin embedded (rat liver tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (H4 (Acetyl K5)) Polyclonal Antibody, Unconjugated (TMAB-07136) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Nucleus. Chromosome. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. [provided by RefSeq, Jul 2008] |
| Immunogen | KLH conjugated synthesised acetylpeptide: human Histone H4 around the acetylation site of Acetyl Lys5 |
| Antigen Species | Human |
| Gene Name | H4C1 |
| Gene ID | |
| Protein Name | Histone H4 |
| Uniprot ID | |
| Function | Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. |
| Molecular Weight | Theoretical: 11 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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