Anti-Acetyl-Histone H4 (Lys12) Antibody (1M287) is a Mouse antibody targeting Acetyl-Histone H4 (Lys12). Anti-Acetyl-Histone H4 (Lys12) Antibody (1M287) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.

IgG1, Kappa

1M287

Human,Mouse,Rat

1. Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:1000
Primary ab incubation condition: 4°C overnight
Secondary ab: Goat Anti-Mouse IgG H&L (HRP)
Lysate: HeLa+BSA (+), HeLa+BSA (-)
Protein loading quantity: 20 μg
Exposure time: 10 s
Predicted MW: 11 kDa
Observed MW: 11 kDa
2. Tissue: Human colon cancer
Section type: Formalin fixed & Paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: SP Kit (Mouse)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for TMAB-02220
3. Tissue: Mouse liver
Section type: Formalin fixed & Paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: SP Kit (Mouse)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for TMAB-02220
4. Tissue: Rat stomach
Section type: Formalin fixed & Paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: SP Kit (Mouse)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for TMAB-02220
5. Cell line: (A) HeLa, (B) HeLa+Trichostatin A (500 ng/ml, 4 h)
Fixative: 4% Paraformaldehyde
Permeabilization: 0.1% TritonX-100
Primary ab dilution: 1:300
Primary incubation condition: 4°C overnight
Secondary ab: Goat Anti-Mouse IgG
Nuclear counter stain: DAPI (Blue)
Counter stain: Tubulin (Red)
Comment: Color green is the positive signal for TMAB-02220

WB: 1:500-1000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:100-500; IF: 1:100-500

Monoclonal

H4C1

Histone H4

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.