Anti-Acetyl-Histone H2B (Lys20) Polyclonal Antibody 2 is a Rabbit antibody targeting Acetyl-Histone H2B (Lys20). Anti-Acetyl-Histone H2B (Lys20) Polyclonal Antibody 2 can be used in IHC-P,IHC-Fr,IF.

IgG

Rat (predicted:Human,Mouse,Dog,Cow,Rabbit)

1. Tissue/cell: rat colon tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-Histone H2B Polyclonal Antibody, Unconjugated (TMAB-07082) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
2. Tissue/cell: rat testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-Histone H2B (Acetyl K20) Polyclonal Antibody, Unconjugated (TMAB-07082) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
3. Tissue/cell: rat colon tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-Histone H2B (Acetyl K20) Polyclonal Antibody, Unconjugated (TMAB-07082) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining

IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated Synthesised acetylpeptide: human Histone H2B around the acetylation site of Lys20

Human

H2BC3

Histone H2B type 1-B

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.