Anti-Acetyl-Histone H2B (Lys20) Antibody (1F585) is a Mouse antibody targeting Acetyl-Histone H2B (Lys20). Anti-Acetyl-Histone H2B (Lys20) Antibody (1F585) can be used in WB,IHC-P,IHC-Fr,IF.

IgG

1F585

Human (predicted:Mouse,Rat)

1. Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1: 2000
Primary ab incubation condition: 2 hours at room temperature
Secondary ab: Goat Anti-Mouse IgG H&L (HRP)
Lysate: (-) HeLa, (+) HeLa+Sodium butyrate (30 mM, 4 h)
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 14 kDa
Observed MW: 14 kDa
2. Cell type: HeLa+Serum starvation (12 h)+SBA (5 mM, 24 h)
Cross-linking conditions: No cross-linking
Amount of chromatin per IP: 5×106 cells
Amount of Ab per IP: 4 μg
Beads type and amount per IP: 50 μL of Protein A/G MagBeads
Comment: The ChIP was performed with 1 μg of normal rabbit IgG as a negative control. Real time quantitative PCR was performed on immunoprecipitated DNA using primers specific for the human GAPDH CDS region, RPL30 Exon 3, FOXO3a-promoter and FOXO3a-downstream. Data are presented as enrichment of each sample relative to total amount of input chromatin at each amplicon.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Monoclonal

KLH conjugated synthetic peptide: human Histone H2B (Acetyl K20)

Human

H2BC3

Histone H2B type 1-B

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.