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Anti-Acetyl-Alpha Tubulin (Lys40) Antibody (1U664) is a Mouse antibody targeting Acetyl-Alpha Tubulin (Lys40). Anti-Acetyl-Alpha Tubulin (Lys40) Antibody (1U664) can be used in WB.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $220 | 7-10 days | |
100 μL | $374 | 7-10 days | |
200 μL | $527 | 7-10 days |
Description | Anti-Acetyl-Alpha Tubulin (Lys40) Antibody (1U664) is a Mouse antibody targeting Acetyl-Alpha Tubulin (Lys40). Anti-Acetyl-Alpha Tubulin (Lys40) Antibody (1U664) can be used in WB. |
Ig Type | IgG |
Clone | 1U664 |
Reactivity | Human,Mouse,Rat,Zebrafish |
Verified Activity | 1. Sample: Lane 1: A549 (Human) Cell Lysate at 30 μg Lane 2: LOVO (Human) Cell Lysate at 30 μg Lane 3: A431 (Human) Cell Lysate at 30 μg Lane 4: Hela (Human) Cell Lysate at 30 μg Primary: Anti-alpha Tubulin (Acetyl Lys40) (TMAB-00033) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 52 kDa Observed band size: 52 kDa 2. 25 μg total protein per Lane of various lysates probed with alpha Tubulin monoclonal antibody, unconjugated (TMAB-00033) at 1:2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min. |
Application | WB |
Recommended Dose | WB: 1:500-2000 |
Antibody Type | Monoclonal |
Host Species | Mouse |
Subcellular Localization | Cytoplasm, cytoskeleton. |
Construction | Hybridoma Monoclonal Antibody |
Purification | Protein G purified |
Appearance | Liquid |
Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
Research Background | Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulin. The genes encoding these microtubule constituents are part of the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes and they are highly conserved among and between species. This gene encodes an alpha tubulin that is a highly conserved homolog of a rat testis-specific alpha tubulin. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2013] |
Immunogen | KLH conjugated synthesised acetylpeptide: human alpha Tubulin around the acetylation site of Acetyl Lys40 |
Antigen Species | Human |
Gene ID | |
Uniprot ID | |
Biology Area | Tubulin |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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