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Anti-ACADM Polyclonal Antibody is a Rabbit antibody targeting ACADM. Anti-ACADM Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $223 | 7-10 days | 7-10 days | |
| 100 μL | $371 | 7-10 days | 7-10 days | |
| 200 μL | $527 | 7-10 days | 7-10 days |
| Description | Anti-ACADM Polyclonal Antibody is a Rabbit antibody targeting ACADM. Anti-ACADM Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Synonyms | mitochondrial antibody, Medium chain specific acyl CoA dehydrogenase mitochondrial, Medium chain specific acyl CoA dehydrogenase, Medium chain fatty acyl CoA dehydrogenase, Medium chain acyl CoA dehydrogenase, MCADH, MCAD, FLJ99884, FLJ93013, FLJ18227, Acyl coenzyme A dehydrogenase C 4 to C 12 straight chain, Acyl coenzyme A dehydrogenase, ACADM_HUMAN, ACAD1, ACAD 1 |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat (predicted:Chicken,Dog,Pig,Cow,Horse,Rabbit) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (human liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (ACADM) Polyclonal Antibody, Unconjugated (TMAB-02183) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 2. Sample: Lane 1: Mouse Liver tissue lysates Lane 2: Mouse Stomach tissue lysates Lane 3: Mouse Heart tissue lysates Lane 4: Rat Heart tissue lysates Lane 5: Human K562 cell lysates Lane 6: Human Hela cell lysates Lane 7: Human THP-1 cell lysates Primary: Anti-ACADM (TMAB-02183) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 47 kDa Observed band size: 47 kDa |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Mitochondrion matrix. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | This gene encodes the medium-chain specific (C4 to C12 straight chain) acyl-Coenzyme A dehydrogenase. The homotetramer enzyme catalyzes the initial step of the mitochondrial fatty acid beta-oxidation pathway. Defects in this gene cause medium-chain acyl-CoA dehydrogenase deficiency, a disease characterized by hepatic dysfunction, fasting hypoglycemia, and encephalopathy, which can result in infantile death. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Immunogen | KLH conjugated synthetic peptide: human ACADM |
| Antigen Species | Human |
| Gene Name | ACADM |
| Gene ID | |
| Protein Name | Medium-chain specific acyl-CoA dehydrogenase, mitochondrial |
| Uniprot ID | |
| Function | This enzyme is specific for acyl chain lengths of 4 to 16. |
| Molecular Weight | Theoretical: 47 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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