Anti-ABO Antibody (3I775) is a Rabbit antibody targeting ABO. Anti-ABO Antibody (3I775) can be used in FCM, IP, WB.

IgG

3I775

Human,Mouse,Rat

1. 25 ug total protein per lane of various lysates (see on figure) probed with ABO monoclonal antibody, unconjugated (TMAB-02177) at 1: 1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. The K562 (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-ABO antibody (TMAB-02177, 1: 100); Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.

FCM=1:50-100; IP=1:20-50; WB=1:500-2000

Monoclonal

A synthesized peptide: human ABO

Human

ABO

Histo-blood group ABO system transferase

This protein is the basis of the ABO blood group system. The histo-blood group ABO involves three carbohydrate antigens: A, B, and H. A, B, and AB individuals express a glycosyltransferase activity that converts the H antigen to the A antigen (by addition of UDP-GalNAc) or to the B antigen (by addition of UDP-Gal), whereas O individuals lack such activity.