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Anti-ABHD14B Antibody (9W382)
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Catalog No. TMAY-02667
Alias HEL-S-299, CIB, abhydrolase domain containing 14B
Anti-ABHD14B Antibody (9W382) is a Rabbit antibody targeting ABHD14B. Anti-ABHD14B Antibody (9W382) can be used in WB,IP.
Anti-ABHD14B Antibody (9W382)
Anti-ABHD14B Antibody (9W382)
😃Good
Catalog No. TMAY-02667Alias HEL-S-299, CIB, abhydrolase domain containing 14B
Anti-ABHD14B Antibody (9W382) is a Rabbit antibody targeting ABHD14B. Anti-ABHD14B Antibody (9W382) can be used in WB,IP.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 100 μL | $270 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Monoclonal Application: WB, IP Reactivity: Human |
| Alias | HEL-S-299, CIB, abhydrolase domain containing 14B |
| Ig Type | Rabbit IgG |
| Clone | 9W382 |
| Reactivity | Human |
| Verified Activity | 1. Anti-ABHD14B rabbit monoclonal antibody at 1:500 dilution. -Lane A: Jurkat Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:22 kDa. -Observed band size:23 kDa. 2. ABHD14B was immunoprecipitated using: -Lane A:0.5 mg Jurkat Whole Cell Lysate. -Lane B:0.5 mg Hela Whole Cell Lysate -0.5 µL anti-ABHD14B rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose. -Primary antibody: -Anti-ABHD14B rabbit monoclonal antibody, at 1:500 dilution. -Secondary antibody: -Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. -Developed using the odyssey technique. -Performed under reducing conditions. -Predicted band size: 25 kDa. -Observed band size: 25 kDa |
| Application | WB,IP |
| Recommended Dose | WB: 1:500-1:1000; IP: 0.2-1 μL/mg of lysate |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | This antibody was obtained from a rabbit immunized with purified, recombinant Human ABHD14B (rh ABHD14B; TMPY-02978; Q96IU4-1; Met1-Gln210). |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | ABHD14B belongs to the AB hydrolase superfamily, ABHD14 family. It can be detected in spleen, thymus, prostate, testis, ovary, small intestine, colon, peripheral blood leukocyte, heart, placenta, lung, liver, skeletal muscle, pancreas and kidney. ABHD14B has hydrolase activity towards p-nitrophenyl butyrate (in vitro) and may interact with TAF1. It may activate transcription. Recombinant human ABHD14B protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. ABHD14B contains an alpha/beta hydrolase fold, which is a catalytic domain found in a very wide range of enzymes. In molecular biology, the alpha/beta hydrolase fold is common to a number of hydrolytic enzymes of widely differing phylogenetic origin and catalytic function. The Ab hydrolase domain containing gene subfamily is comprised of 15 mostly uncharacterized members. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein: Human ABHD14B protein (TMPY-02978) |
| Antigen Species | Human |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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