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Anti-ABCF1 Antibody (8X278) is a Rabbit antibody targeting ABCF1. Anti-ABCF1 Antibody (8X278) can be used in ICC/IF,IHC,WB.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $297 | 7-10 days | |
100 μL | $497 | 7-10 days |
Description | Anti-ABCF1 Antibody (8X278) is a Rabbit antibody targeting ABCF1. Anti-ABCF1 Antibody (8X278) can be used in ICC/IF,IHC,WB. |
Synonyms | zgc:85667, wu:fj94a08, wu:fc39a05, wu:fb79c06, TNF-alpha-stimulated ABC protein, TNFalpha inducible ATP binding protein, TNF alpha stimulated ABC protein, GCN20, EST123147, D17Wsu166e, AU041969, ATP-binding cassette sub-family F member 1, ATP-binding cassette 50, ATP binding cassette, sub family F (GCN20), member 1, ATP binding cassette sub family F member 1, ATP binding cassette 50 (TNF alpha stimulated), ATP binding cassette 50, ABCF1_HUMAN, ABCF 1, ABC50, ABC27 |
Ig Type | IgG |
Clone | 8X278 |
Reactivity | Human,Rat |
Verified Activity | 1. Western blot analysis of ABCF1 on Hela lysates using anti-ABCF1 antibody at 1/1,000 dilution. 2. Immunohistochemical analysis of paraffin-embedded rat stomach tissue using anti-ABCF1 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-ABCF1 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-ABCF1 antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-ABCF1 antibody. Counter stained with hematoxylin. 6. ICC staining ABCF1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. ICC staining ABCF1 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 8. ICC staining ABCF1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
Application | |
Recommended Dose | WB: 1:1000-2000; IHC: 1:50-200; ICC/IF: 1:100-500 |
Antibody Type | Monoclonal |
Host Species | Rabbit |
Construction | Recombinant Antibody |
Purification | ProA affinity purified |
Appearance | Liquid |
Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
Research Background | ABCF1 (ATP-binding cassette sub-family F member 1, TNF-alpha-stimulated ABC protein) is a 845 amino acid protein encoded by the human gene ABCF1. ABCF1 belongs to the ABC transporter family (EF3 subfamily) and contains two ABC transporter domains. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). Unlike other members of the superfamily, this protein lacks the transmembrane domains which are characteristic of most ABC transporters. ABCF1 is believed to have a role in mRNA translation due to its interaction with eukaryotic initiation factor 2 (eIF2). It is also associated with ribosomes. ABCF1 is ubiquitously expressed and can be induced with TNF. Upon DNA damage, ABCF1 is phosphorylated by either ATM or ATR. |
Conjucates | Unconjugated |
Immunogen | A synthesized peptide: within aa 796-845 of Human ABCF1 |
Antigen Species | human |
Uniprot ID |
Molecular Weight | Theoretical: 96 kDa. |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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