Anti-ABCE1 Antibody (3T571) is a Rabbit antibody targeting ABCE1. Anti-ABCE1 Antibody (3T571) can be used in WB,ICC/IF,IHC-P,IHC-Fr,IF,FCM,IP.

IgG/Kappa

3T571

Human,Mouse,Rat

1. Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ABCE1 Monoclonal Antibody, Unconjugated (TMAB-02157) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ABCE1 Monoclonal Antibody, Unconjugated (TMAB-02157) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ABCE1 Monoclonal Antibody, Unconjugated (TMAB-02157) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
4. 25 μg total protein per lane of various lysates (see on figure) probed with ABCE1 monoclonal antibody, unconjugated (TMAB-02157) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
5. The Hela (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-ABCE1 antibody (TMAB-02157, 1:100); Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.

WB: 1:500-2000; ICC/IF: 1:50-200; IHC-P: 1:50-200; IHC-Fr: 1:50-200; IF: 1:50-200; FCM: 1:50-100; IP: 1:20-50

Monoclonal

A synthesized peptide: human ABCE1

ABCE1

ATP-binding cassette sub-family E member 1

Antagonizes the binding of 2-5A (5'-phosphorylated 2',5'-linked oligoadenylates) by RNase L through direct interaction with RNase L and therefore inhibits its endoribonuclease activity. May play a central role in the regulation of mRNA turnover. Antagonizes the anti-viral effect of the interferon-regulated 2-5A/RNase L pathway. May act as a chaperone for post-translational events during HIV-1 capsid assembly.