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Anti-ABCB5 Antibody (9D935) (Synonyms: P glycoprotein ABCB5, EST422562, ATP-binding cassette sub-family B memb...)
Catalog No. TMAB-02146 Copy Product Info
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Anti-ABCB5 Antibody (9D935) is a Mouse antibody targeting ABCB5. Anti-ABCB5 Antibody (9D935) can be used in WB,IHC-P,IHC-Fr,IF.
Anti-ABCB5 Antibody (9D935)
Copy Product Info🥰Excellent
Catalog No. TMAB-02146
Synonyms P glycoprotein ABCB5, EST422562, ATP-binding cassette sub-family B member 5, ATP binding cassette sub family B(MDR/TAP) member 5, ATP binding cassette sub family B member 5, ABCB5beta, ABCB5alpha, ABCB5_HUMAN, ABCB 5
Anti-ABCB5 Antibody (9D935) is a Mouse antibody targeting ABCB5. Anti-ABCB5 Antibody (9D935) can be used in WB,IHC-P,IHC-Fr,IF.
Anti-ABCB5 Antibody
(9D935)
(9D935)
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 50 μL | $221 | 7-10 days | 7-10 days | |
| 100 μL | $371 | 7-10 days | 7-10 days | |
| 200 μL | $527 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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| Description | Anti-ABCB5 Antibody (9D935) is a Mouse antibody targeting ABCB5. Anti-ABCB5 Antibody (9D935) can be used in WB,IHC-P,IHC-Fr,IF. |
| Synonyms | P glycoprotein ABCB5, EST422562, ATP-binding cassette sub-family B member 5, ATP binding cassette sub family B(MDR/TAP) member 5, ATP binding cassette sub family B member 5, ABCB5beta, ABCB5alpha, ABCB5_HUMAN, ABCB 5 |
| Ig Type | IgG |
| Clone | 9D935 |
| Reactivity | Human |
| Verified Activity | 1. Sample: Lane 1: Human HepG2 cell lysates Lane 2: Human HL-60 cell lysates Primary: Anti-ABCB5 (TMAB-02146) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 89 kDa Observed band size: 85 kDa 2. Paraformaldehyde-fixed, paraffin embedded (human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (ABCB5) Monoclonal Antibody, Unconjugated (TMAB-02146) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (ABCB5) Monoclonal Antibody, Unconjugated (TMAB-02146) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded (human kidney carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (ABCB5) Monoclonal Antibody, Unconjugated (TMAB-02146) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining. |
| Application | |
| Recommended Dose | WB: 1:500-1000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Subcellular Localization | Cell membrane; Multi-pass membrane protein. |
| Tissue Specificity | Expressed by CD133-expressing progenitor cells among epidermal melanocytes (at protein level). Widely expressed with specific expression in pigment cells. Also expressed in several malignant tissues. |
| Construction | Hybridoma Monoclonal Antibody |
| Purification | Protein G purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | ABCB5 belongs to the ATP-binding cassette (ABC) transporter superfamily of integral membrane proteins. These proteins participate in ATP-dependent transmembrane transport of structurally diverse molecules ranging from small ions, sugars, and peptides to more complex organic molecules (Chen et al., 2005 [PubMed 15760339]).[supplied by OMIM, Mar 2008] |
| Immunogen | KLH conjugated synthetic peptide: human ABCB5 |
| Antigen Species | Human |
| Gene Name | ABCB5 |
| Gene ID | |
| Protein Name | ATP-binding cassette sub-family B member 5 |
| Uniprot ID | |
| Function | Plasma membrane transporter able to mediate efflux from cells of the rhodamine dye and of the therapeutic drug doxorubicin. Responsible for the resistance to doxorubicin of a subset of malignant melanomas. |
| Molecular Weight | Theoretical: 89 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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