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Anti-A3AR Polyclonal Antibody is a Rabbit antibody targeting A3AR. Anti-A3AR Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $220 | 7-10 days | |
100 μL | $372 | 7-10 days | |
200 μL | $528 | 7-10 days |
Description | Anti-A3AR Polyclonal Antibody is a Rabbit antibody targeting A3AR. Anti-A3AR Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM. |
Alias | TGPCR1, RP11 552M11.7, bA552M11.5, ADORA3, ADORA 3, adenosine receptor A3 isoform 2, adenosine receptor A3 isoform 1, adenosine A3 receptor, AD026, AD 026, AA3R |
Ig Type | IgG |
Reactivity | Human,Mouse,Rat (predicted:Dog,Pig,Cow,Rabbit,Sheep) |
Verified Activity | 1. Tissue/cell: human gastric carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-ADORA3 Polyclonal Antibody, Unconjugated (TMAB-00017) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 2. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Adenosine A3 Receptor) Polyclonal Antibody, Unconjugated (TMAB-00017) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining. 3. Sample: Lane 1: Heart (Mouse) Tissue Lysate at 40 μg Lane 2: Bone (Mouse) Tissue Lysate at 40 μg Lane 3: Heart (Rat) Tissue Lysate at 40 μg Primary: Anti-Adenosine A3 Receptor (TMAB-00017) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 35 kDa Observed band size: 36 kDa 4. Blank control: HL-60. Primary Antibody (green line): Rabbit Anti-Adenosine A3 Receptor antibody (TMAB-00017) Dilution: 2 μg/10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-FITC Dilution: 1 μg/test. Protocol The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. 5. Blank control (blue line): Raji (blue)(The cells were fixed with 70% ethanol overnight at-20°C). Primary Antibody (green line): Rabbit Anti-Adenosine A3 Receptor antibody (TMAB-00017); Dilution: 1 μg/10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE;Dilution: 1 μg/test. |
Application | WB,IHC-P,IHC-Fr,IF,FCM |
Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1μg /test |
Antibody Type | Polyclonal |
Host Species | Rabbit |
Subcellular Localization | Cell membrane. |
Construction | Hybridoma Polyclonal Antibody |
Purification | Protein A purified |
Appearance | Liquid |
Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
Research Background | This gene encodes a protein that belongs to the family of adenosine receptors, which are G-protein-coupled receptors that are involved in a variety of intracellular signaling pathways and physiological functions. The receptor encoded by this gene mediates a sustained cardioprotective function during cardiac ischemia, it is involved in the inhibition of neutrophil degranulation in neutrophil-mediated tissue injury, it has been implicated in both neuroprotective and neurodegenerative effects, and it may also mediate both cell proliferation and cell death. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]. |
Immunogen | KLH conjugated synthetic peptide: human ADORA3 |
Antigen Species | Human |
Gene ID | |
Uniprot ID | |
Biology Area | More GPCR,Heart disease,Cardiac metabolism,Ischemia / Reperfusion |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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