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Anti-A Raf Polyclonal Antibody
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Catalog No. TMAB-02116
Alias v raf oncogene homolog 1(murine sarcoma 3611 virus), v raf murine sarcoma 3611 viral oncogene homolog 1, v raf murine sarcoma 3611 viral oncogene homolog, Ras binding protein DA Raf, RAFA1, RAFA 1, Raf1, Proto oncogene Pks, PKS2, PKS 2, PKS, Oncogene PKS2, Oncogene Araf1, ARAF1, ARaf proto oncogene serine/threonine protein kinase, ARAF 1, A-Raf, ARAF, A Raf proto oncogene serine/threonine protein kinase, A raf 1
Anti-A Raf Polyclonal Antibody is a Rabbit antibody targeting A Raf. Anti-A Raf Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-A Raf Polyclonal Antibody
Anti-A Raf Polyclonal Antibody
Copy Product Info😃Good
Catalog No. TMAB-02116Alias v raf oncogene homolog 1(murine sarcoma 3611 virus), v raf murine sarcoma 3611 viral oncogene homolog 1, v raf murine sarcoma 3611 viral oncogene homolog, Ras binding protein DA Raf, RAFA1, RAFA 1, Raf1, Proto oncogene Pks, PKS2, PKS 2, PKS, Oncogene PKS2, Oncogene Araf1, ARAF1, ARaf proto oncogene serine/threonine protein kinase, ARAF 1, A-Raf, ARAF, A Raf proto oncogene serine/threonine protein kinase, A raf 1
Anti-A Raf Polyclonal Antibody is a Rabbit antibody targeting A Raf. Anti-A Raf Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $221 | 7-10 days | 7-10 days | |
| 100 μL | $371 | 7-10 days | 7-10 days | |
| 200 μL | $529 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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| Description | Anti-A Raf Polyclonal Antibody is a Rabbit antibody targeting A Raf. Anti-A Raf Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Synonyms | v raf oncogene homolog 1(murine sarcoma 3611 virus), v raf murine sarcoma 3611 viral oncogene homolog 1, v raf murine sarcoma 3611 viral oncogene homolog, Ras binding protein DA Raf, RAFA1, RAFA 1, Raf1, Proto oncogene Pks, PKS2, PKS 2, PKS, Oncogene PKS2, Oncogene Araf1, ARAF1, ARaf proto oncogene serine/threonine protein kinase, ARAF 1, A-Raf, ARAF, A Raf proto oncogene serine/threonine protein kinase, A raf 1 |
| Ig Type | IgG |
| Reactivity | Human,Rat (predicted:Mouse) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (Rat bladder); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (A Raf) Polyclonal Antibody, Unconjugated (TMAB-02116) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 2. Sample: 293T (Human) Cell Lysate at 30 μg Raji (Human) Cell Lysate at 30 μg Primary: Anti-A Raf (TMAB-02116) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 68 kD Observed band size: 65 kD 3. Sample: Hela (Human) Cell Lysate at 30 μg NIH/3T3 (Mouse) Cell Lysate at 30 μg Primary: Anti-A Raf (TMAB-02116) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 68 kD Observed band size: 65 kD 4. Paraformaldehyde-fixed, paraffin embedded (Human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (A Raf) Polyclonal Antibody, Unconjugated (TMAB-02116) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Tissue Specificity | Predominantly in urogenital tissues. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | The RAF family of signaling proteins has three isoforms: A RAF, RAF 1 and B RAF. These proteins are instrumental in forwarding the stimulus generated by mitogens from monomeric G proteins to the MAP kinase pathway. RAF 1 is known to be downstream of Ras and binds to it by an N terminal noncatalytic domain. This domain is conserved in A RAF and B RAF. 14-3-3 is also known to be an activator of RAFs. Activated RAF directly phosphorylates and activates MEK. A RAF is expressed at a very high concentration in urogenital and kidney tissues. |
| Immunogen | KLH conjugated synthetic peptide: human A Raf/ARAF |
| Antigen Species | Human |
| Gene Name | ARAF |
| Gene ID | |
| Protein Name | Serine/threonine-protein kinase A-Raf |
| Uniprot ID | |
| Function | Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. |
| Molecular Weight | Theoretical: 68 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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