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Anti-A-RAF Antibody (1K508)
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Catalog No. TMAB-02802
Anti-A-RAF Antibody (1K508) is a Rabbit antibody targeting A-RAF. Anti-A-RAF Antibody (1K508) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM.
Anti-A-RAF Antibody (1K508)
Anti-A-RAF Antibody (1K508)
Copy Product Info😃Good
Catalog No. TMAB-02802
Anti-A-RAF Antibody (1K508) is a Rabbit antibody targeting A-RAF. Anti-A-RAF Antibody (1K508) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $262 | 7-10 days | 7-10 days | |
| 100 μL | $470 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
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| Description | Anti-A-RAF Antibody (1K508) is a Rabbit antibody targeting A-RAF. Anti-A-RAF Antibody (1K508) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM. |
| Ig Type | IgG |
| Clone | 1K508 |
| Reactivity | Human |
| Verified Activity | 1. 25 μg total protein per lane of various lysates (see on figure) probed with A-RAF monoclonal antibody, unconjugated (TMAB-02802) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min. 2. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with A-RAF Monoclonal Antibody, Unconjugated (TMAB-02802) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining. |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:50-200; IHC-Fr: 1:50-200; ICC/IF: 1:50-200; IF: 1:50-200; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Tissue Specificity | Predominantly in urogenital tissues. |
| Construction | Recombinant Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | The RAF family of signaling proteins has three isoforms: A RAF, RAF 1 and B RAF. These proteins are instrumental in forwarding the stimulus generated by mitogens from monomeric G proteins to the MAP kinase pathway. RAF 1 is known to be downstream of Ras and binds to it by an N terminal noncatalytic domain. This domain is conserved in A RAF and B RAF. 14-3-3 is also known to be an activator of RAFs. Activated RAF directly phosphorylates and activates MEK. A RAF is expressed at a very high concentration in urogenital and kidney tissues. |
| Immunogen | Recombinant Protein: human A-RAF protein (150-300aa) |
| Antigen Species | Human |
| Gene Name | ARAF |
| Gene ID | |
| Protein Name | Serine/threonine-protein kinase A-Raf |
| Uniprot ID | |
| Function | Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. |
| Molecular Weight | Theoretical: 68 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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