Anti-5HT4 Receptor Polyclonal Antibody is a Rabbit antibody targeting 5HT4 Receptor. Anti-5HT4 Receptor Polyclonal Antibody can be used in IF, IHC-Fr, IHC-P, WB.

IgG

Mouse,Rat (predicted:Human,Chicken,Cow)

1. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (5HT4 Receptor) Polyclonal Antibody, Unconjugated (TMAB-00012) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
2. Sample:
Lane 1: Stomach (Mouse) Tissue Lysate at 40 μg
Lane 2: Cerebrum (Mouse) Tissue Lysate at 40 μg
Lane 3: Heart (Mouse) Tissue Lysate at 40 μg
Lane 4: Adrenal gland (Mouse) Tissue Lysate at 40 μg
Lane 5: Adrenal gland (Rat) Tissue Lysate at 40 μg
Primary: Anti-5HT4 Receptor (TMAB-00012) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 43 kDa
Observed band size: 48 kDa
3. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (5HT4 Receptor) Polyclonal Antibody, Unconjugated (TMAB-00012) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (5HT4 Receptor) Polyclonal Antibody, Unconjugated (TMAB-00012) at 1:400 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody for 90 minutes, and DAPI for nucleus staining.
5. Sample:
Large intestine (Mouse) Lysate at 40 μg
Primary: Anti-5HT4 Receptor (TMAB-00012) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 43 kDa
Observed band size: 43 kDa
6. Sample:
Bone (Mouse) Lysate at 40 μg
Primary: Anti-5HT4 Receptor at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 43 kDa
Observed band size: 43 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:200-800

Polyclonal

KLH conjugated synthetic peptide: human 5-HTR4

Human

HTR4

5-hydroxytryptamine receptor 4

Obesity,Serotonin Receptors

This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins that stimulate adenylate cyclase.