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Anti-5HT4 Receptor Polyclonal Antibody
😃Good
Catalog No. TMAB-00012
Alias Serotonin receptor 4, Serotonin 5 HT4 receptor, Htr4a, HTR4, HTR 4, Cardiac 5 HT4 receptor, 5-HTR4(5-hydroxytryptamine(serotonin) receptor 4a), 5HT4R, 5-HT4A, 5-HT4, 5HT4, 5 hydroxytryptamine serotonin receptor 4, 5 hydroxytryptamine receptor 4, 5 hydroxytryptamine 4 receptor, 5 HT4R, 5 HT4, 5 HT 4
Anti-5HT4 Receptor Polyclonal Antibody is a Rabbit antibody targeting 5HT4 Receptor. Anti-5HT4 Receptor Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-5HT4 Receptor Polyclonal Antibody
Anti-5HT4 Receptor Polyclonal Antibody
😃Good
Catalog No. TMAB-00012Alias Serotonin receptor 4, Serotonin 5 HT4 receptor, Htr4a, HTR4, HTR 4, Cardiac 5 HT4 receptor, 5-HTR4(5-hydroxytryptamine(serotonin) receptor 4a), 5HT4R, 5-HT4A, 5-HT4, 5HT4, 5 hydroxytryptamine serotonin receptor 4, 5 hydroxytryptamine receptor 4, 5 hydroxytryptamine 4 receptor, 5 HT4R, 5 HT4, 5 HT 4
Anti-5HT4 Receptor Polyclonal Antibody is a Rabbit antibody targeting 5HT4 Receptor. Anti-5HT4 Receptor Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $221 | 7-10 days | |
| 100 μL | $373 | 7-10 days | |
| 200 μL | $527 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-5HT4 Receptor Polyclonal Antibody is a Rabbit antibody targeting 5HT4 Receptor. Anti-5HT4 Receptor Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Alias | Serotonin receptor 4, Serotonin 5 HT4 receptor, Htr4a, HTR4, HTR 4, Cardiac 5 HT4 receptor, 5-HTR4(5-hydroxytryptamine(serotonin) receptor 4a), 5HT4R, 5-HT4A, 5-HT4, 5HT4, 5 hydroxytryptamine serotonin receptor 4, 5 hydroxytryptamine receptor 4, 5 hydroxytryptamine 4 receptor, 5 HT4R, 5 HT4, 5 HT 4 |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat (predicted:Chicken,Cow) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (5HT4 Receptor) Polyclonal Antibody, Unconjugated (TMAB-00012) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 2. Sample: Lane 1: Stomach (Mouse) Tissue Lysate at 40 μg Lane 2: Cerebrum (Mouse) Tissue Lysate at 40 μg Lane 3: Heart (Mouse) Tissue Lysate at 40 μg Lane 4: Adrenal gland (Mouse) Tissue Lysate at 40 μg Lane 5: Adrenal gland (Rat) Tissue Lysate at 40 μg Primary: Anti-5HT4 Receptor (TMAB-00012) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 43 kDa Observed band size: 48 kDa 3. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (5HT4 Receptor) Polyclonal Antibody, Unconjugated (TMAB-00012) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (5HT4 Receptor) Polyclonal Antibody, Unconjugated (TMAB-00012) at 1:400 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody for 90 minutes, and DAPI for nucleus staining. 5. Sample: Large intestine (Mouse) Lysate at 40 μg Primary: Anti-5HT4 Receptor (TMAB-00012) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 43 kDa Observed band size: 43 kDa 6. Sample: Bone (Mouse) Lysate at 40 μg Primary: Anti-5HT4 Receptor at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 43 kDa Observed band size: 43 kDa |
| Application | WB,IHC-P,IHC-Fr,IF |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:200-800 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cell membrane; Multi-pass membrane protein. Endosome. Note=Interaction with SNX27 mediates recruitment to early endosomes, while interaction with SLC9A3R1 and EZR might target the protein to specialized subcellular regions, such as microvilli. |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | This gene is a member of the family of serotonin receptors, which are G protein coupled receptors that stimulate cAMP production in response to serotonin (5-hydroxytryptamine). The gene product is a glycosylated transmembrane protein that functions in both the peripheral and central nervous system to modulate the release of various neurotransmitters. Multiple transcript variants encoding proteins with distinct C-terminal sequences have been described. [provided by RefSeq, May 2010] |
| Immunogen | KLH conjugated synthetic peptide: human 5-HTR4 |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Obesity,Serotonin Receptors |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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