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Anti-5HT1D Receptor Polyclonal Antibody is a Rabbit antibody targeting 5HT1D Receptor. Anti-5HT1D Receptor Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $223 | 7-10 days | 7-10 days | |
| 100 μL | $371 | 7-10 days | 7-10 days | |
| 200 μL | $527 | 7-10 days | 7-10 days |
| Description | Anti-5HT1D Receptor Polyclonal Antibody is a Rabbit antibody targeting 5HT1D Receptor. Anti-5HT1D Receptor Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Synonyms | SR-1D, Serotonin receptor 1D, S12, RDC4, HTRL, HTR1DA, HTR1D2, HTR1D, HT1DA, 5HT1D_HUMAN, 5HT1D, 5 hydroxytryptamine(serotonin) receptor 1D G protein coupled, 5 hydroxytryptamine(serotonin) receptor 1B, 5 hydroxytryptamine receptor 1D, 5 hydroxytryptamine 1B receptor(5 HT 1B)(Serotonin receptor)(5 HT 1D beta)(Serotonin 1D beta receptor)(S12), 5 HT1D, 5 HT1B, 5 HT 1D alpha, 5 HT 1D |
| Ig Type | IgG |
| Reactivity | Mouse,Rat (predicted:Human,Rabbit) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (5HT1D Receptor) Polyclonal Antibody, Unconjugated (TMAB-02099) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 2. Sample: Lane 1: Mouse Cerebrum tissue lysates Lane 2: Rat Spinal cord tissue lysates Primary: Anti-5HT1D Receptor (TMAB-02099) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 42 kDa Observed band size: 46 kDa |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cell membrane; Multi-pass membrane protein |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | The members of the G-protein-coupled receptor family are distinguished by their slow transmitting response to ligand binding. These seven transmembrane proteins include the adrenergic, serotonin and dopamine receptors. The effect of the signaling molecule can be excitatory or inhibitory depending on the type of receptor to which it binds. b-adrenergic bound to adrenaline activates adenylyl cyclase, while a2-adrenergic receptor bound to adrenaline inhibits adenylyl cyclase. Like the a2-adrenergic receptor, serotonin receptor functions are also mediated by G proteins that inhibit the activity of adenylyl cyclase. The serotonin receptors have been classified into several categories, designated SR-1–7 (5HT1–7). Subtypes within the SR-1 group include SR-1A, -1B, -1D, -1E and -1F. |
| Immunogen | KLH conjugated synthetic peptide: human 5HT1D Receptor/SR-1D |
| Antigen Species | Human |
| Gene Name | HTR1D |
| Gene ID | |
| Protein Name | 5-hydroxytryptamine receptor 1D |
| Uniprot ID | |
| Function | This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins that inhibit adenylate cyclase activity. |
| Molecular Weight | Theoretical: 42 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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