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Anti-5-BrdU Antibody (4C731)
😃Good
Catalog No. TMAB-00006
Anti-5-BrdU Antibody (4C731) is a Mouse antibody targeting 5-BrdU. Anti-5-BrdU Antibody (4C731) can be used in ICC/IF.
Anti-5-BrdU Antibody (4C731)
Anti-5-BrdU Antibody (4C731)
😃Good
Catalog No. TMAB-00006
Anti-5-BrdU Antibody (4C731) is a Mouse antibody targeting 5-BrdU. Anti-5-BrdU Antibody (4C731) can be used in ICC/IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $221 | 7-10 days | |
| 100 μL | $374 | 7-10 days | |
| 200 μL | $529 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Antibody Type: Mouse Monoclonal Application: ICC/IF Reactivity: Species independent |
| Ig Type | IgG |
| Clone | 4C731 |
| Reactivity | Species independent |
| Verified Activity | 4% Paraformaldehyde-fixed Hela (treated with 10uM BrdU for 24 hours) (H) cells; 2M HCL at RT for 20 min; Antibody incubation with (BrdU) monoclonal Antibody, unconjugated (TMAB-00006) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Mouse IgG antibody (green) at 37°C for 90 min, DAPI (blue) was used to stain the cell nucleus. |
| Application | ICC/IF |
| Recommended Dose | ICC/IF: 1:100 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Subcellular Localization | Nuclear. |
| Construction | Hybridoma Monoclonal Antibody |
| Purification | Protein G purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthezised DNA of S-phase cells may provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information such as the S-phase transit rate and the potential doubling time can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis |
| Immunogen | KLH conjugated Brdu |
| Biology Area | Markers,DNA / Nucleotides,Soma marker,Replication |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
Sci Citations
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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