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Anti-4EBP1 Polyclonal Antibody
😃Good
Catalog No. TMAB-00004
Anti-4EBP1 Polyclonal Antibody is a Rabbit antibody targeting 4EBP1. Anti-4EBP1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-4EBP1 Polyclonal Antibody
Anti-4EBP1 Polyclonal Antibody
😃Good
Catalog No. TMAB-00004
Anti-4EBP1 Polyclonal Antibody is a Rabbit antibody targeting 4EBP1. Anti-4EBP1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $222 | 7-10 days | |
| 100 μL | $373 | 7-10 days | |
| 200 μL | $527 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-4EBP1 Polyclonal Antibody is a Rabbit antibody targeting 4EBP1. Anti-4EBP1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat (predicted:Dog,Cow,Horse,Goat,Sheep) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with eIF4EBP1 Polyclonal Antibody, Unconjugated (TMAB-00004) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 2. Sample: Lane 1: Human HepG2 cell lysates Lane 2: Human HeLa cell lysates Primary: Anti-eIF4EBP1 (TMAB-00004) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 12 kDa Observed band size: 17 kDa |
| Application | WB,IHC-P,IHC-Fr,IF |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Nucleus. |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation. [provided by RefSeq, Jul 2008]. |
| Immunogen | KLH conjugated synthetic peptide: human 4E-BP1 |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | RNAi,Regulation,Eukaryotic Initiation factors (eIF's),Diabetes,Metabolic disorders,Obesity |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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