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Anti-4EBP1 Antibody (5U577) (Synonyms: PHAS-I, eukaryotic translation initiation factor 4E binding protein 1, ...)

Catalog No. TMAC-01276 Copy Product Info
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Anti-4EBP1 Antibody (5U577) is a Rabbit antibody targeting 4EBP1. Anti-4EBP1 Antibody (5U577) can be used in FCM,ICC/IF,IHC,IP,WB.

Anti-4EBP1 Antibody (5U577)

Copy Product Info
🥰Excellent
Catalog No. TMAC-01276
Synonyms PHAS-I, eukaryotic translation initiation factor 4E binding protein 1, BP-1, 4E-BP1, 4EBP1

Anti-4EBP1 Antibody (5U577) is a Rabbit antibody targeting 4EBP1. Anti-4EBP1 Antibody (5U577) can be used in FCM,ICC/IF,IHC,IP,WB.

Anti-4EBP1 Antibody
(5U577)
Pack SizePriceUSA StockGlobal StockQuantity
50 μL$2977-10 days7-10 days
100 μL$4967-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-4EBP1 Antibody (5U577) is a Rabbit antibody targeting 4EBP1. Anti-4EBP1 Antibody (5U577) can be used in FCM,ICC/IF,IHC,IP,WB.
SynonymsPHAS-I, eukaryotic translation initiation factor 4E binding protein 1, BP-1, 4E-BP1, 4EBP1
Ig Type
IgG
Clone
5U577
Reactivity
Human,Mouse,Rat
Verified Activity
1. Western blot analysis of eIF4EBP1 on different lysates using anti-eIF4EBP1 antibody at 1/1,000 dilution. Positive control: Lane 1: K562, Lane 2: HepG2, Lane 3: 293.
2. Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-eIF4EBP1 antibody. Counter stained with hematoxylin.
3. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-eIF4EBP1 antibody. Counter stained with hematoxylin.
4. ICC staining eIF4EBP1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
5. ICC staining eIF4EBP1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
6. Flow cytometric analysis of NIH/3T3 cells with eIF4EBP1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
verifiedActivityverifiedActivityverifiedActivityverifiedActivityverifiedActivityverifiedActivity
Application
Recommended Dose
WB: 1:1000-2000; IHC: 1:50-200; ICC/IF: 1:50-200; FCM: 1:50-100
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionRecombinant Antibody
PurificationProA affinity purified
AppearanceLiquid
Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
Research BackgroundThe translation of proteins from eukaryotic mRNA is initiated by the multisubunit complex eIF-4F, which associates with the mRNA 5' cap structure. eIF-4E, a component of eIF-4F, is responsible for binding to the 5' cap structure and for the assembly of the eIF-4F complex. The regulatory protein 4E-BP1, also referred to as PHAS-I, inhibits eIF-4E function. Phosphorylation of 4E-BP1 by S6 kinase p70, MAP kinases or PKCs causes the disassociation of 4E-BP1 from eIF-4E, promoting translation. A protein that is functionally related to 4E-BP1, designated 4E-BP2, also associates with eIF-4E.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Protein
Uniprot ID
Chemical Properties
Molecular WeightTheoretical: 17 kDa.
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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