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Anti-4EBP1 Antibody (2G39)

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Catalog No. TMAY-00091
Alias PHAS-I, eukaryotic translation initiation factor 4E binding protein 1, BP-1, 4E-BP1, 4EBP1

Anti-4EBP1 Antibody (2G39) is a Rabbit antibody targeting 4EBP1. Anti-4EBP1 Antibody (2G39) can be used in ICC/IF.

Anti-4EBP1 Antibody (2G39)

Anti-4EBP1 Antibody (2G39)

😃Good
Catalog No. TMAY-00091Alias PHAS-I, eukaryotic translation initiation factor 4E binding protein 1, BP-1, 4E-BP1, 4EBP1
Anti-4EBP1 Antibody (2G39) is a Rabbit antibody targeting 4EBP1. Anti-4EBP1 Antibody (2G39) can be used in ICC/IF.
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100 μL$270 7-10 days
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Product Introduction

Bioactivity
Description
Anti-4EBP1 Antibody (2G39) is a Rabbit antibody targeting 4EBP1. Anti-4EBP1 Antibody (2G39) can be used in ICC/IF.
AliasPHAS-I, eukaryotic translation initiation factor 4E binding protein 1, BP-1, 4E-BP1, 4EBP1
Ig Type
Monoclonal Rabbit IgG
Clone
2G39
Reactivity
Human
Verified Activity
Immunofluorescence staining of Human 4EBP1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human 4EBP1 monoclonal antibody (1:60) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to nucleus and cytoplasm.
Application
ICC/IF
Recommended Dose
ICC-IF: 1:20-1:100
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionThis antibody was obtained from a rabbit immunized with purified, recombinant Human 4E-BP1 / EIF4EBP1 (rh 4E-BP1 / EIF4EBP1; TMPY-01769; NP_004086.1; Ser2-Ile118).
PurificationProtein A
AppearanceLiquid
Formulation0.2 μm filtered solution in PBS
Research BackgroundThe translational suppressor eIF4E binding protein-1, 4E-BP1 functions as a key regulator in cellular growth, differentiation, apoptosis and survival. The Eif4ebp1 gene, encoding 4E-BP1, is a direct target of a transcription factor activating transcription factor-4 (ATF4), a master regulator of gene expression in stress responses. 4E-BP1 is characterized by its capacity to bind specifically to eIF4E and inhibit its interaction with eIF4G. Phosphorylation of 4E-BP1 regulates eIF4E availability, and therefore, cap-dependent translation, in cell stress. Binding of eIF4E to eIF4G is inhibited in a competitive manner by 4E-BP1. Phosphorylation of 4E-BP1 decreases the affinity of this protein for eIF4E, thus favouring the binding of eIF4G and enhancing translation. 4E-BP1 is important for beta-cell survival under endoplasmic reticulum (ER) stress. 4E-BP1 mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways. Recently, 4E-BP1 was found to be a key factor, which converges several oncogenic signals, phosphorylates the molecules, and drives the downstream proliferative signals. Recent studies showed that high expression of phosphorylated 4E-BP-1 (p-4E-BP1) is associated with poor prognosis, tumor progression, or nodal metastasis in different human cancers.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Human 4E-BP1 / EIF4EBP1 protein (TMPY-01769)
Antigen Species
Human
Chemical Properties
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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