Anti-14-3-3 zeta+delta Polyclonal Antibody is a Rabbit antibody targeting 14-3-3 zeta+delta. Anti-14-3-3 zeta+delta Polyclonal Antibody can be used in WB,FCM.

IgG

Human,Mouse,Rat (predicted:Chicken,Dog,Cow,Horse,Rabbit)

1. Sample:
Cerebrum (Mouse) Lysate at 40 μg
Primary: Anti-14-3-3 zeta+deltA (TMAB-02090) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 27 kD
Observed band size: 27 kD
2. Blank control (blue line): HL60 (fixed with 70% ethanol (overninght at 4℃) and then permeabilized with 0.1% P Dilution: 0.2 μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE
3. Sample:
Cerebrum (Rat) Lysate at 40 μg
Primary: Anti-14-3-3 zeta+delta (TMAB-02090) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 27 kD
Observed band size: 27 kD
4. Blank control (black line): Raji (black) (The cells were fixed with 2% paraformaldehyde (10 min), then permeabilized with PBST for 30 min on room temperature)
Primary Antibody (rad line): Rabbit Anti-14-3-3 zeta+delta antibody (TMAB-02090);
Dilution: 1 μg /10^6 cells;
Isotype Control Antibody (grenn line): Rabbit IgG.
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE; Dilution: 1 μg /test.

WB: 1:500-2000; FCM: 1μg/Test

Polyclonal

KLH conjugated synthetic peptide: human 14-3-3 zeta/delta

Human

YWHAZ

14-3-3 protein zeta/delta

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.