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Anti-14-3-3/YWHAZ Antibody (1K428) is a Mouse antibody targeting 14-3-3/YWHAZ. Anti-14-3-3/YWHAZ Antibody (1K428) can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $296 | 7-10 days | 7-10 days | |
| 100 μL | $472 | 7-10 days | 7-10 days |
| Description | Anti-14-3-3/YWHAZ Antibody (1K428) is a Mouse antibody targeting 14-3-3/YWHAZ. Anti-14-3-3/YWHAZ Antibody (1K428) can be used in WB,IHC-P,IHC-Fr,IF. |
| Synonyms | YWHAD, Tyrosine 3-Monooxygenase/Tryptophan 5-Monooxygenase Activation Protein, Zeta Polypeptide, Tyrosine 3-Monooxygenase/Tryptophan 5-Monooxygenase Activation Protein, delta Polypeptide, Tyrosine 3-Monooxygenase/Tryptophan 5-Monooxygenase Activation Protein Zeta, Tyrosine 3/Tryptophan 5-Monooxygenase Activation Protein, Zeta Polypeptide, protein kinase C inhibitor protein-1, Protein kinase C inhibitor protein 1, phospholipase A2, KCIP-1, HEL-S-93, HEL-S-3, Epididymis Secretory Protein Li 93, Epididymis Secretory Protein Li 3, Epididymis Luminal Protein 4, 14-3-3-Zeta, 14-3-3 zeta, 14-3-3 Protein/Cytosolic phospholipase A2, 14-3-3 protein zeta/delta, 14-3-3 delta |
| Ig Type | IgG2b |
| Clone | 1K428 |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (YWHAZ) Polyclonal Antibody, Unconjugated (TMAB-02091) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining. 2. Sample: Hela (Human) Cell Lysate at 40 μg A549 (Human) Cell Lysate at 40 μg Cerebrum (Rat) Lysate at 40 μg Panc-1 (Human) Lysate at 40 μg Primary: Anti-YWHAZ at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 23 kD Observed band size: 23 kD |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Subcellular Localization | Cytoplasm. Melanosome. Note=Located to stage I to stage IV melanosomes. |
| Construction | Hybridoma Monoclonal Antibody |
| Purification | Protein G purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. |
| Immunogen | Recombinant Protein: human YWHAZ protein |
| Antigen Species | Human |
| Gene Name | YWHAZ |
| Gene ID | |
| Protein Name | 14-3-3 protein zeta/delta |
| Uniprot ID | |
| Function | Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. |
| Molecular Weight | Theoretical: 27 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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