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Anti-14‑3‑3 theta/YWHAQ Antibody (5O606)
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Catalog No. TMAC-00008
Alias tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, θ, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta, HS1, 41701, 1C5
Anti-14‑3‑3 theta/YWHAQ Antibody (5O606) is a Rabbit antibody targeting 14‑3‑3 theta/YWHAQ. Anti-14‑3‑3 theta/YWHAQ Antibody (5O606) can be used in FCM,ICC/IF,WB.
Anti-14‑3‑3 theta/YWHAQ Antibody (5O606)
Anti-14‑3‑3 theta/YWHAQ Antibody (5O606)
😃Good
Catalog No. TMAC-00008Alias tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, θ, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta, HS1, 41701, 1C5
Anti-14‑3‑3 theta/YWHAQ Antibody (5O606) is a Rabbit antibody targeting 14‑3‑3 theta/YWHAQ. Anti-14‑3‑3 theta/YWHAQ Antibody (5O606) can be used in FCM,ICC/IF,WB.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $296 | 7-10 days | 7-10 days | |
| 100 μL | $496 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Chemical Properties
| Description | Anti-14‑3‑3 theta/YWHAQ Antibody (5O606) is a Rabbit antibody targeting 14‑3‑3 theta/YWHAQ. Anti-14‑3‑3 theta/YWHAQ Antibody (5O606) can be used in FCM,ICC/IF,WB. |
| Synonyms | tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, θ, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta, HS1, 41701, 1C5 |
| Ig Type | IgG |
| Clone | 5O606 |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Western blot analysis of 14-3-3 Theta on different lysates using anti-14-3-3 Theta antibody at 1/1,000 dilution. Positive control: Lane 1: Hela, Lane 2: MCF-7. 2. ICC staining 14-3-3 Theta in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 3. ICC staining 14-3-3 Theta in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 4. ICC staining 14-3-3 Theta in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 5. Flow cytometric analysis of SH-SY-5Y cells with 14-3-3 Theta antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.      |
| Application | |
| Recommended Dose | WB: 1:1000-5000; ICC/IF: 1:100-500; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | 14-3-3 proteins regulate many cellular processes relevant to cancer biology, notably apoptosis, mitogenic signaling and cell-cycle checkpoints. Seven isoforms comprise this family of signaling intermediates, denoted 14-3-3 β, γ, ε, ζ, η, θ and ??. 14-3-3 proteins form dimers that present two binding sites for ligand proteins, thereby bringing together two proteins that may not otherwise associate. These ligands largely share a 14-3-3 consensus binding motif and exhibit serine/threonine phosphorylation. 14-3-3 proteins function in broad regulation of these ligand proteins, by cytoplasmic sequestration, occupation of interaction domains and import/export sequences, prevention of degradation, activation/repression of enzymatic activity and facilitation of protein modification, and thus loss of expression contributes to a vast array of pathogenic cellular activities. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 28 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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