Anti-14-3-3 sigma Antibody (7Y889) is a Mouse antibody targeting 14-3-3 sigma. Anti-14-3-3 sigma Antibody (7Y889) can be used in WB,IHC-P,IHC-Fr,IF.

IgG1, Kappa

7Y889

Human

1. Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:1000
Primary ab incubation condition: room temperature 2 h
Secondary ab: Goat Anti-Mouse IgG H&L (HRP)
Lysate: HeLa, MCF-7
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 28 kDa
Observed MW: 28 kDa
2. Tissue: Human esophagus
Section type: Formalin fixed & Paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: SP Kit (Mouse)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for TMAB-02086
3. Tissue: Human tonsil
Section type: Formalin fixed & Paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: SP Kit (Mouse)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for TMAB-02086

WB: 1:500-2000; IHC-P: 1:50-100; IHC-Fr: 1:400-800; IF: 1:100-500

Monoclonal

KLH conjugated synthetic peptide: human 14-3-3 sigma

Human

SFN

14-3-3 protein sigma

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway.
p53-regulated inhibitor of G2/M progression.