Anti-14-3-3 epsilon Antibody (1W307) is a Rabbit antibody targeting 14-3-3 epsilon. Anti-14-3-3 epsilon Antibody (1W307) can be used in WB,IHC-P,IHC-Fr,IF.

IgG

1W307

Human,Mouse,Rat

1. 25 μg total protein per lane of various lysates (see on figure) probed with 14-3-3 epsilon monoclonal antibody, unconjugated (TMAB-02079) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with 14-3-3 epsilon Monoclonal Antibody, Unconjugated (TMAB-02079) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with 14-3-3 epsilon Monoclonal Antibody, Unconjugated (TMAB-02079) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with 14-3-3 epsilon Monoclonal Antibody, Unconjugated (TMAB-02079) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with 14-3-3 epsilon Monoclonal Antibody, Unconjugated (TMAB-02079) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.

WB: 1:500-2000; IHC-P: 1:50-200; IHC-Fr: 1:50-200; IF: 1:50-200

Monoclonal

A synthesized peptide: human 14-3-3 epsilon

Human

YWHAE

14-3-3 protein epsilon

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.