CD32A Protein, Human, Recombinant (R167, His)

Catalog No. TMPJ-00446

Synonyms: FcRII-a, IGFR2, Low Affinity Immunoglobulin Gamma Fc Region Receptor II-a, Fc-Gamma RII-a, FCGR2A1, Fc-γ RII-a, FCGR2A, CDw32, FCG2, Fc-Gamma-RIIa, CD32, Low Affinity Immunoglobulin γ Fc Region Receptor II-a, IgG Fc receptor II-a, Fc-γ-RIIa

Receptors for the Fc region of IgG (FcγR) are members of the Ig superfamily that function in the activation or inhibition of immune responses. Human FcγRs are divided into three classes designated FcγRI (CD64), FcγRII (CD32), and FcγRIII (CD16), which generate multiple isoforms, are recognized. The activating type receptor either has or associates noncovalently with an accessory subunit that has an immunoreceptor tyrosinebased activation motif (ITAM) in its cytoplasmic domain. FcγRI binds IgG with high affinity and functions during early immune responses, whereas FcγRII and RIII are low affinity receptors that recognize IgG as aggregates surrounding multivalent antigens during late immune responses. Three genes for human FcγRII (A, B, and C) and one for mouse (FcγRIIB), encoding type I transmembrane proteins with ITAM motifs (FcγRII A and C) or ITIM motifs (FcγRIIB) in their cytoplasmic domains, have been identified. Human CD32, also known as Low affinity immunoglobulin γ Fc region receptor II-a (IgG Fc receptor II-a), FcγRII A or FCGR2A Protein, is expressed on cells of both myeloid and lymphoid lineages as well as on cells of non-hematopoietic origin. Associated with an ITAM-bearing adapter subunit, FcRγ, CD32a (FcγRII A) delivers an activating signal upon ligand binding, and results in the initiation of inflammatory responses including cytolysis, phagocytosis, degranulation, and cytokine production. The responses can be modulated by signals from the co-expressed inhibitory receptors such as Fcγ RII B, and the strength of the signal is dependent on the ratio of expression of the activating and inhibitory receptors.

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CD32A Protein, Human, Recombinant (R167, His)

Pack Size	Availability	Price/USD
10 μg	5 days	$ 97.00
50 μg	5 days	$ 272.00
500 μg	5 days	$ 1,900.00
1 mg	5 days	$ 2,730.00

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DATASHEET SDS

Biological Description

Technical Params

Product Properties

Description

Species	Human
Expression System	HEK293 Cells
Tag	C-6xHis
Accession Number	AAA35827
Synonyms	FcRII-a, IGFR2, Low Affinity Immunoglobulin Gamma Fc Region Receptor II-a, Fc-Gamma RII-a, FCGR2A1, Fc-γ RII-a, FCGR2A, CDw32, FCG2, Fc-Gamma-RIIa, CD32, Low Affinity Immunoglobulin γ Fc Region Receptor II-a, IgG Fc receptor II-a, Fc-γ-RIIa
Amino Acid	Ala36-Ile218
Construction	Ala36-Ile218. It is identical to FCGR2A131H/R in the reference.
Protein Purity	Greater than 95% as determined by reducing SDS-PAGE. Greater than 95% as determined by SEC-HPLC.
Molecular Weight	25-32 KDa (reducing condition)

Endotoxin	< 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM PB, 150 mM NaCl, pH 7.4.
Reconstitution	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage	Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted protein solutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.
Research Background	Receptors for the Fc region of IgG (FcγR) are members of the Ig superfamily that function in the activation or inhibition of immune responses. Human FcγRs are divided into three classes designated FcγRI (CD64), FcγRII (CD32), and FcγRIII (CD16), which generate multiple isoforms, are recognized. The activating type receptor either has or associates noncovalently with an accessory subunit that has an immunoreceptor tyrosinebased activation motif (ITAM) in its cytoplasmic domain. FcγRI binds IgG with high affinity and functions during early immune responses, whereas FcγRII and RIII are low affinity receptors that recognize IgG as aggregates surrounding multivalent antigens during late immune responses. Three genes for human FcγRII (A, B, and C) and one for mouse (FcγRIIB), encoding type I transmembrane proteins with ITAM motifs (FcγRII A and C) or ITIM motifs (FcγRIIB) in their cytoplasmic domains, have been identified. Human CD32, also known as Low affinity immunoglobulin γ Fc region receptor II-a (IgG Fc receptor II-a), FcγRII A or FCGR2A Protein, is expressed on cells of both myeloid and lymphoid lineages as well as on cells of non-hematopoietic origin. Associated with an ITAM-bearing adapter subunit, FcRγ, CD32a (FcγRII A) delivers an activating signal upon ligand binding, and results in the initiation of inflammatory responses including cytolysis, phagocytosis, degranulation, and cytokine production. The responses can be modulated by signals from the co-expressed inhibitory receptors such as Fcγ RII B, and the strength of the signal is dependent on the ratio of expression of the activating and inhibitory receptors.

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Keywords

CD32A Protein, Human, Recombinant (R167, His) FcRII-a IGFR2 Low Affinity Immunoglobulin Gamma Fc Region Receptor II-a Fc-Gamma RII-a FCGR2A1 Fc-γ RII-a IGFR 2 FCGR2A CDw32 FCG2 Fc-Gamma-RIIa CD32 Low Affinity Immunoglobulin γ Fc Region Receptor II-a CD 32 IgG Fc receptor II-a IGFR-2 Fc-γ-RIIa FCG 2 FCG-2 CD-32 recombinant recombinant-proteins proteins protein