Powder: -20°C for 3 years | In solvent: -80°C for 1 year
PAC-1 (Procaspase activating compound 1) has been used in trials studying the treatment of Lymphoma, Melanoma, Solid Tumors, Breast Cancer, and Thoracic Cancers, among others.
Pack Size | Availability | Price/USD | Quantity |
---|---|---|---|
10 mg | In stock | $ 43.00 | |
25 mg | In stock | $ 75.00 | |
50 mg | In stock | $ 125.00 | |
100 mg | In stock | $ 226.00 | |
500 mg | In stock | $ 913.00 | |
1 mL * 10 mM (in DMSO) | In stock | $ 30.00 |
Description | PAC-1 (Procaspase activating compound 1) has been used in trials studying the treatment of Lymphoma, Melanoma, Solid Tumors, Breast Cancer, and Thoracic Cancers, among others. |
Targets&IC50 | Procaspase-3:0.22 μM(EC50) |
Kinase Assay | In vitro procaspase-3 activation: Procaspase-3 is expressed and purified in Escherichia coli. Various concentrations of PAC-1 are added to 90 μL of a 50 ng/mL of procaspase-3 in caspase assay buffer in a 96-well plate, The plate is incubated for 12 hours at 37 °C. A 10 μL volume of a 2 mM solution of caspase-3 peptidic substrate acetyl Asp-Glu-Val-Asp-p-nitroanilide (Ac-DEVD-pNa) in caspase assay buffer is then added to each well. The plate is read every 2 minutes at 405 nm for 2 hours in a Spectra Max Plus 384 well plate reader. The slope of the linear portion for each well is determined, and the relative increase in activation from untreated control wells is calculated. |
Cell Research | Cells are exposed to various concentrations of PAC-1 for 72 hours. Cell death is quantified by the addition of MTS/PMS CellTiter 96 Cell Proliferation Assay reagent. The plates are incubated at 37 °C for approximately 1 hour (until the colored product formed), and the absorbance is measured at 490 n(Only for Reference) |
Synonyms | Procaspase activating compound 1 |
Molecular Weight | 392.49 |
Formula | C23H28N4O2 |
CAS No. | 315183-21-2 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
1eq. HCl: 29.4 mg/mL (75 mM)
DMSO: 55 mg/mL (140.13 mM)
You can also refer to dose conversion for different animals. More
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