VCAM-1 Protein, Human, Recombinant (His)

Name: VCAM-1 Protein, Human, Recombinant (His)
Brand: TargetMol
SKU: TMPY-00948
Rating: 4.5 (1 reviews)

(Synonyms: VCAM-1, vascular cell adhesion molecule 1, INCAM-100, CD106) Copy Product Info

Synonyms: VCAM-1, vascular cell adhesion molecule 1, INCAM-100, CD106

Catalog No. TMPY-00948 Copy Product Info

VCAM-1 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 76 kDa and the accession number is P19320-1.

Pack Size	Price	USA Stock	Global Stock
5 µg	$48	7-10 days	7-10 days
10 µg	$76	7-10 days	7-10 days
20 µg	$118	7-10 days	7-10 days
50 µg	$237	7-10 days	7-10 days
100 µg	$458	7-10 days	7-10 days
200 µg	$693	7-10 days	7-10 days
500 µg	$1,450	7-10 days	7-10 days
1 mg	$2,930	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells. When cells are added to VCAM1 coated plates (5 μg/mL, 100 μL/well) approximately 60% will adhere after 1 hour of incubation at 37℃.
Description	VCAM-1 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 76 kDa and the accession number is P19320-1.
Species	Human
Expression System	HEK293 Cells
Tag	N-His
Accession Number	P19320-1
Construction	A DNA sequence encoding the extracellular domain (Phe 25-Pro 697) of human VCAM1 (NP_001069.1) was fused with a polyhistidine tag at the N-terminus. Predicted N terminal: His
Protein Purity	> 95 % as determined by SDS-PAGE
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Synonyms	VCAM-1, vascular cell adhesion molecule 1, INCAM-100, CD106
Research Background	Vascular cell adhesion molecule 1 (VCAM-1), also known as CD106, is a cell surface sialoglycoprotein belonging to the immunoglobulin superfamily. Two forms of VCAM-1 with either six or seven extracellular Ig-like domains are generated by alternative splicing, with the longer form predominant. VCAM-1 is an endothelial ligand for very late antigen-4 (VLA-4) and α4ß7 integrin expressed on leukocytes, and thus mediates leukocyte-endothelial cell adhesion and signal transduction. VCAM-1 expression is induced on endothelial cells during inflammatory bowel disease, atherosclerosis, allograft rejection, infection, and asthmatic responses. During these responses, VCAM-1 forms a scaffold for leukocyte migration. VCAM-1 also activates signals within endothelial cells resulting in the opening of an "endothelial cell gate" through which leukocytes migrate. VCAM-1 has been identified as a potential anti-inflammatory therapeutic target, the hypothesis being that reduced expression of VCAM-1 will slow the development of atherosclerosis. In addition, VCAM-1-activated signals in endothelial cells are regulated by cytokines indicating that it is important to consider both endothelial cell adhesion molecule expression and function during inflammatory processes.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy

Chemical Properties

Molecular Weight

76 kDa (predicted); 110 kDa (reducing condition, due to glycosylation)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.