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Synonyms: Tumor necrosis factor ligand superfamily member 2, TNFSF2, TNF-alpha, TNF-a, TNFA, DIF, Cachectin


| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 5 µg | $71 | - | In Stock | |
| 10 µg | $113 | - | In Stock | |
| 20 µg | $168 | - | In Stock | |
| 50 µg | $297 | - | In Stock | |
| 100 µg | $439 | - | In Stock |
| Bioactivity | ED 50 < 50.0 pg/ml, measured in a cytotoxicity assay using mouse L-929 cells in the presence of actinomycin D, corresponding to a specific activity of > 2.0 × 10 7 units/mg. |
| Description | TNF alpha Protein, Rat, Recombinant (Yeast) is expressed in P. pastoris (Yeast). The accession number is P06804. |
| Species | Rat |
| Expression System | P. pastoris (Yeast) |
| Tag | Tag Free |
| Accession Number | P06804 |
| Construction | Leu80-Leu235 |
| Protein Purity | > 95% as determined by SDS-PAGE; > 95% as determined by HPLC |
| Endotoxin | < 0.2 EU/μg of protein as determined by the LAL method. |
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute the lyophilized protein in sterile deionized water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
| Synonyms | Tumor necrosis factor ligand superfamily member 2, TNFSF2, TNF-alpha, TNF-a, TNFA, DIF, Cachectin |
| Research Background | Tumor Necrosis Factor-Alpha (TNF-alpha) plays a major role in growth regulation, differentiation, inflammation, viral replication, tumorigenesis, and autoimmune disease. Besides inducing hemorrhagic necrosis of tumors, TNF has been found to be involved in tumorigenesis, tumor metastasis, viral replication, septic shock, fever, inflammation, and autoimmune disease including Crohn's disease, rheumatoid arthritis and graft-versus-host disease. TNF alpha-1a is a potent lymphoid factor that exerts cytotoxic effects on a wide range of tumor cells and certain other target cells. |
| Molecular Weight | ~17.4 kDa (Reducing conditions) |
| Shipping | In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice. |
| Storage | Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles. |
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