SARS-COV-2 Spike S1 NTD Protein (His & Flag)

Catalog No. TMPK-00444

The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. SARS-COV-2 Spike S1 NTD Protein (His & Flag) is expressed in HEK293 mammalian cells with C-His-Flag tag. The predicted molecular weight is 35 kDa and the accession number is A0A6G7K2L4.

SARS-COV-2 Spike S1 NTD Protein (His & Flag)

Catalog No. TMPK-00444

Pack Size	Price	USA Warehouse	Global Warehouse
5 μg	$61	7-10 days	7-10 days
10 μg	$96	7-10 days	7-10 days
20 μg	$156	7-10 days	7-10 days
50 μg	$297	7-10 days	7-10 days
100 μg	$511	7-10 days	7-10 days
200 μg	$925	7-10 days	7-10 days
500 μg	$2,030	7-10 days	7-10 days

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Expected start date of shipmentUSA Warehouse [1-2 days] Global Warehouse [5-7 days]

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Product Information

Biological Activity	Activity has not been tested. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
Description	The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. SARS-COV-2 Spike S1 NTD Protein (His & Flag) is expressed in HEK293 mammalian cells with C-His-Flag tag. The predicted molecular weight is 35 kDa and the accession number is A0A6G7K2L4.
Species	SARS-CoV-2
Expression System	HEK293 Cells
Tag	C-His-Flag
Accession Number	P0DTC2
Synonyms	S1 protein NTD,Spike glycoprotein Subunit1 NTD,S glycoprotein Subunit1 NTD,Spike protein S1 NTD
Construction	Val16-Ser305
Protein Purity	> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Molecular Weight	35 kDa (predicted). Due to glycosylation, the protein migrates to 55-70 kDa based on Tris-Bis PAGE result.
Endotoxin	< 1 EU/μg by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

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