It is easy for the proteins to be degraded or dephosphorylated during in vitro extraction procedure, which could cause an inaccurate result of protein expression detection. Therefore, adding protease inhibitors or phosphatase inhibitors into the extracts would be an effective method to prevent degradation and dephosphorylation of protein.
The phosphatase inhibitor mixture can effectively inhibit the dephosphorylation of common phosphatases on proteins and maintain the original phosphorylation state of proteins.
|(-)-p-Bromotetramisole oxalate||62284-79-1||373.22||2.5 mM||Alkaline phosphatases||Irreversible|
|Cantharidin||56-25-7||196.2||500 μM||Ser/Thr phosphatases||Reversible|
|Microcystin LR||101043-37-2||995.17||500 nM||Acid and PP1 and PP2A||Reversible|
|Formulation||In 1 ml DMSO|
|Storage||-20 ℃, Avoid free/thaw|
|Freeze||Ok to freeze|