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Pulsatilla saponin D

Name: Pulsatilla saponin D
Brand: TargetMol
SKU: T3909
Price: 329 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T3909Cas No. 68027-15-6

Alias SB365, Hederacolchiside A

Pulsatilla saponin D (Hederacolchiside A) has exhibited potential beneficial effects as a chemopreventive agent for critical health conditions including cancer, and it inhibits cell growth via cell cycle arrest and apoptosis in various cancer cell types.

Pulsatilla saponin D

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Purity: 99.55%

Catalog No. T3909Alias SB365, Hederacolchiside ACas No. 68027-15-6

Pack Size	Price	USA Warehouse	Global Warehouse
1 mg	$59	In Stock	In Stock
5 mg	$135	In Stock	In Stock
10 mg	$198	In Stock	In Stock
25 mg	$329	In Stock	In Stock
50 mg	Inquiry	In Stock	In Stock
1 mL x 10 mM (in DMSO)	$250	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Batch Information

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Purity:99.55%

Appearance:Solid

Color:White

Resource Download

COA LCMS HPLC HNMR

Product Introduction

Pulsatilla saponin D AI Summary

Pulsatilla saponin D exhibits multiple bioactivities, including potent molluscicidal activity against Biomphalaria alexandrina, achieving 100% activity at 20 ppm and 90% activity at 10 ppm within 48 hours. It shows antifungal activity against Candida albicans (MIC ≤ 16.0 µg/mL) and Cryptococcus neoformans (MIC ≤ 8.0 µg/mL), but not against Aspergillus fumigatus (MIC ≥ 250.0 µg/mL). This compound also demonstrates cytotoxicity against various human cell lines such as A549, DLD1, WS1, Bel7402, SMMC7721, HL7702, and HL-60, with IC50 values ranging from 3.8 µg/mL to 62,000 nM, and exhibits a cytotoxic effect on human L02 cells with an IC50 of 21,120 nM. Cytotoxic effects include MTT assay responses, cell membrane permeabilization, and induction of apoptosis and cell cycle arrest. Additionally, Pulsatilla saponin D displays hemolytic activity in erythrocytes from different origins, with HD50 values ranging from 6.3 µM to 22.0 µM. Furthermore, the compound has oil-water and apparent partition coefficients of logP = 0.1149 and logPapp = 1.303, respectively..

Note: Summary generated by AI. Data source: ChEMBL

Bioactivity

Description	Pulsatilla saponin D (Hederacolchiside A) has exhibited potential beneficial effects as a chemopreventive agent for critical health conditions including cancer, and it inhibits cell growth via cell cycle arrest and apoptosis in various cancer cell types.
Synonyms	SB365, Hederacolchiside A

Chemical Properties

Molecular Weight	913.1
Formula	C₄₇H₇₆O₁₇
Cas No.	68027-15-6
Smiles	[H][C@@]1(O[C@H]2CC[C@@]3(C)[C@@]([H])(CC[C@]4(C)[C@]3([H])CC=C3[C@]5([H])CC(C)(C)CC[C@@]5(CC[C@@]43C)C(O)=O)[C@]2(C)CO)OC[C@H](O[C@]2([H])O[C@H](CO)[C@@H](O)[C@H](O)[C@H]2O)[C@H](O)[C@H]1O[C@]1([H])O[C@@H](C)[C@H](O)[C@@H](O)[C@H]1O
Relative Density.	1.40 g/cm3 at 20℃

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 40 mg/mL (43.81 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 2 mg/mL (2.19 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	1.0952 mL	5.4759 mL	10.9517 mL	54.7585 mL
5 mM	0.2190 mL	1.0952 mL	2.1903 mL	10.9517 mL
10 mM	0.1095 mL	0.5476 mL	1.0952 mL	5.4759 mL
20 mM	0.0548 mL	0.2738 mL	0.5476 mL	2.7379 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc