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Neriifolin

Name: Neriifolin
Brand: TargetMol
SKU: T124830
Price: 148 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T124830Cas No. 466-07-9

Alias NSC123976, NSC 123976, 17β-Neriifolin, 17beta-Neriifolin

Neriifolin (17β-Neriifolin), a cardiac glycoside extracted from the unripe fruit of Cerbera manghas, is a Na+K+-ATPase inhibitor with anticancer activity that inhibits cell proliferation by suppressing HOXA9-dependent gene expression and inducing apoptosis in the human acute myeloid cell line, THP-1. Neriifolin induces cell cycle arrest and apoptosis in human hepatocellular carcinoma HepG2 cells and can be used in prostate cancer research.

Neriifolin

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Catalog No. T124830Alias NSC123976, NSC 123976, 17β-Neriifolin, 17beta-NeriifolinCas No. 466-07-9

Pack Size	Price	USA Warehouse	Global Warehouse	Quantity
1 mg	$148	-	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Appearance:Solid

Color:White

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COA

Product Introduction

Bioactivity

Description	Neriifolin (17β-Neriifolin), a cardiac glycoside extracted from the unripe fruit of Cerbera manghas, is a Na+K+-ATPase inhibitor with anticancer activity that inhibits cell proliferation by suppressing HOXA9-dependent gene expression and inducing apoptosis in the human acute myeloid cell line, THP-1. Neriifolin induces cell cycle arrest and apoptosis in human hepatocellular carcinoma HepG2 cells and can be used in prostate cancer research.
In vitro	Treatment of Neriifolin at a concentration of 0.1 μg/mL for 48 hours was able to cause apoptosis of HepG2 cells. In addition, Neriifolin significantly reduced the viability of HepG2 cells after 72 hours of treatment at concentrations ranging from 0 to 8 μg/mL. The compound also caused cell cycle arrest in the S and G2/M phases, further promoting apoptosis in HepG2 cells. When HepG2 cells were stimulated by Neriifolin, caspase-3, caspase-8, and caspase-9 were activated, while the expression of Fas and FasL proteins was upregulated. [1]
Synonyms	NSC123976, NSC 123976, 17β-Neriifolin, 17beta-Neriifolin

Chemical Properties

Molecular Weight	534.68
Formula	C₃₀H₄₆O₈
Cas No.	466-07-9
Smiles	O[C@@]12[C@]3([C@@]([C@]4(C)[C@](CC3)(C[C@@H](O[C@H]5[C@@H](O)[C@H](OC)[C@@H](O)[C@H](C)O5)CC4)[H])(CC[C@]1(C)[C@H](CC2)C6=CC(=O)OC6)[H])[H]

Storage & Solubility Information

Storage

keep away from direct sunlight,store at low temperature,keep away from moisture | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 80 mg/mL (149.62 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 3.3 mg/mL (6.17 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	1.8703 mL	9.3514 mL	18.7028 mL	93.5139 mL
5 mM	0.3741 mL	1.8703 mL	3.7406 mL	18.7028 mL
10 mM	0.1870 mL	0.9351 mL	1.8703 mL	9.3514 mL
20 mM	0.0935 mL	0.4676 mL	0.9351 mL	4.6757 mL
50 mM	0.0374 mL	0.1870 mL	0.3741 mL	1.8703 mL
100 mM	0.0187 mL	0.0935 mL	0.1870 mL	0.9351 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc