N-Oleoyl glycine, a lipoamino acid, was able to promote 3T3-L1 adipogenesis through the activation of CB1 receptor and the enhancement of insulin-mediated Akt signaling pathway.

N-Oleoyl glycine stimulated lipid accumulation and significantly increased adipogenic genes (PPARγ and aP2), in a dose- and time-dependent manner.?Additionally, N-Oleoyl glycine markedly increased the mRNA expression of CB1 receptor and the inhibition of CB1R by its antagonist SR141716 abolished the promotive effects of N-Oleoyl glycine on lipid accumulation and the protein expression of PPARγ and aP2.?Furthermore, N-Oleoyl glycine increased the ratio of p-Akt/Akt and p-FoxO1/FoxO1, which could be reversed by SR141716.?Moreover, N-Oleoyl glycine-induced enhancement of adipogenesis, activation of insulin-mediated Akt signaling pathway and inactivation of FoxO1 were effectively blocked by Wortmannin, a specific PI3K/Akt inhibitor, indicating the essential role of Akt signaling pathway in the process of N-Oleoyl glycine-stimulated 3T3-L1 adipogenesis[1].