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Methacholine Chloride

Name: Methacholine Chloride
Brand: TargetMol
SKU: T1682
Price: 32 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T1682Cas No. 62-51-1

Alias Mecholyl chloride, Acetyl-β-methylcholine chloride

Methacholine Chloride (Acetyl-β-methylcholine chloride) is a quaternary ammonium parasympathomimetic agent with the muscarinic actions of ACETYLCHOLINE. It is hydrolyzed by ACETYLCHOLINESTERASE at a considerably slower rate than ACETYLCHOLINE and is more resistant to hydrolysis by nonspecific CHOLINESTERASES so that its actions are more prolonged. It is used as a parasympathomimetic bronchoconstrictor agent and as a diagnostic aid for bronchial asthma.

Methacholine Chloride

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Purity: 99.74%

Catalog No. T1682Alias Mecholyl chloride, Acetyl-β-methylcholine chlorideCas No. 62-51-1

Pack Size	Price	USA Warehouse	Global Warehouse
25 mg	$32	In Stock	In Stock
50 mg	$43	In Stock	In Stock
100 mg	$59	In Stock	In Stock
500 mg	$142	In Stock	In Stock
1 g	$198	In Stock	In Stock
1 mL x 10 mM (in DMSO)	$47	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Batch Information

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Purity:99.74%

Color:White

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COA

Product Introduction

Methacholine Chloride AI Summary

Methacholine Chloride exhibits a wide range of bioactivities. It acts as a potent inhibitor in various assays including those targeting Tau fibril formation, Bloom's syndrome helicase, Histone Lysine Methyltransferase G9a, delayed death of the malarial parasite, Polymerase Iota, Hepatitis C Virus, GLP-1 receptor inverse agonists, human tyrosyl-DNA phosphodiesterase 1, human Apurinic/apyrimidinic Endonuclease 1, and Ebola virus entry. Additionally, it enhances the expression of the SMN2 splice variant and inhibits Nrf2. The compound's potency ranges from nanomolar to micromolar levels, with specific IC50 and AC50 values across various bioassays. It significantly inhibits sodium fluorescein uptake in OATP1B3- and OATP1B1-transfected CHO cells at a 10 μM concentration, with inhibitions of 106.87% and 86.62%, respectively. Furthermore, Methacholine Chloride acts as an agonist at the muscarinic acetylcholine receptor in guinea pig ileum, displaying a pD2 value of 7.02, which denotes its potency in activating this receptor. However, it shows very low activity in inhibiting cell viability in Vero E6 cells infected with SARS-CoV-2, with an inhibition index of 0.009846, indicating minimal effectiveness in this context..

Note: Summary generated by AI. Data source: ChEMBL

Bioactivity

Description

Synonyms

Mecholyl chloride, Acetyl-β-methylcholine chloride

Chemical Properties

Molecular Weight	195.69
Formula	C₈H₁₈ClNO₂
Cas No.	62-51-1
Smiles	[Cl-].CC(C[N+](C)(C)C)OC(C)=O
Relative Density.	1.1028 g/cm3 (Estimated)

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 194 mg/mL (991.36 mM), Sonication is recommended.

H2O: 100 mg/mL (511.01 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 2 mg/mL (10.22 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

H2O/DMSO

	1mg	5mg	10mg	50mg
1 mM	5.1101 mL	25.5506 mL	51.1012 mL	255.5062 mL
5 mM	1.0220 mL	5.1101 mL	10.2202 mL	51.1012 mL
10 mM	0.5110 mL	2.5551 mL	5.1101 mL	25.5506 mL
20 mM	0.2555 mL	1.2775 mL	2.5551 mL	12.7753 mL
50 mM	0.1022 mL	0.5110 mL	1.0220 mL	5.1101 mL
100 mM	0.0511 mL	0.2555 mL	0.5110 mL	2.5551 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc