Prussian blue insoluble

Prussian blue insoluble is an antidote that effectively captures and promotes the excretion of heavy metal ions such as cesium and thallium in the gastrointestinal tract through ion exchange. Additionally, Prussian blue insoluble possesses antibacterial and antitumor activities and can serve as a contrast agent for photoacoustic imaging and magnetic resonance imaging (MRI). Prussian blue insoluble can also be used as an adsorbent for the effective removal of heavy metals (Cu(II), Co(II), Ni(II), and Pb(II)) from aqueous solutions.

Method: Human cervical cancer HeLa cells were treated with PEGylated Prussian blue nanoparticles (PEGylated PB NPs) at concentrations of 50 μg·mL⁻¹ and 100 μg·mL⁻¹, followed by exposure to NIR laser irradiation (808 nm, 2.0 W) for different durations (0, 3, 5 min). Cell killing effects were assessed using live/dead cell staining (calcein-AM / PI) and the MTT assay.
Result: PEGylated PB NPs exhibited no obvious toxicity to HeLa cells in the absence of laser irradiation. After 5 min of NIR laser irradiation, a significant induction of cell death was observed at 50 μg·mL⁻¹, and the killing effect increased with longer irradiation time and higher NP concentration[1].
Method: Escherichia coli suspensions (10–10⁸ CFU/mL) were incubated with insoluble Prussian blue (PBins, 0.07 mM) nanoparticles in MH medium (pH 6.2) for 24 h. The reduction of Prussian blue to Prussian white was monitored by measuring absorbance spectra (400–900 nm) every hour.
Result: At a bacterial concentration of 10⁸ CFU/mL, PBins were completely reduced to colorless Prussian white within <10 h; at a low concentration of 10 CFU/mL, complete decolorization required approximately 20 h[2].