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F(ab')₂ Fragment Goat Anti-rabbit IgG H&L Secondary Antibody-FITC is a FITC-conjugated Goat antibody targeting IgG H&L. F(ab')₂ Fragment Goat Anti-rabbit IgG H&L Secondary Antibody-FITC can be used in FCM,ICC/IF,IF.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
100 μL | $225 | 7-10 days |
Description | F(ab')₂ Fragment Goat Anti-rabbit IgG H&L Secondary Antibody-FITC is a FITC-conjugated Goat antibody targeting IgG H&L. F(ab')₂ Fragment Goat Anti-rabbit IgG H&L Secondary Antibody-FITC can be used in FCM,ICC/IF,IF. |
Synonyms | FITC AffiniPure F(ab')2 Fragment Goat Anti-Rabbit IgG (H+L), F(ab')₂ Fragment Goat Anti-rabbit IgG H+L (FITC), F(ab)2 Fragment Goat Anti-Rabbit IgG H&L/FITC, F(ab)2 Fragment Goat Anti-Rabbit IgG H&L (FITC) |
Ig Type | IgG |
Reactivity | Rabbit |
Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Vimentin Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green, TMAB-02062F), DAPI (blue) was used to stain the cell nuclei.
2. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Vimentin Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green, TMAB-02062F), DAPI (blue) was used to stain the cell nuclei. 3. Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Vimentin Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green, TMAB-02062F), DAPI (blue) was used to stain the cell nuclei. 4. Paraformaldehyde-fixed, paraffin embedded Human Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Vimentin Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green, TMAB-02062F), DAPI (blue) was used to stain the cell nuclei. 5. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Vimentin Monoclonal Antibody, Unconjugated (bsm-42001R) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green, TMAB-02062F), DAPI (blue) was used to stain the cell nuclei. 6. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Vimentin Monoclonal Antibody, Unconjugated (bsm-42001R) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green, TMAB-02062F), DAPI (blue) was used to stain the cell nuclei. 7. Paraformaldehyde-fixed, paraffin embedded Human Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Vimentin Monoclonal Antibody, Unconjugated (bsm-42001R) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green, TMAB-02062F), DAPI (blue) was used to stain the cell nuclei. 8. Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Vimentin Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green, TMAB-02062F), DAPI (blue) was used to stain the cell nuclei. 9. Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Vimentin Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green, TMAB-02062F), DAPI (blue) was used to stain the cell nuclei. ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() |
Application | |
Recommended Dose | IF: 1:200-1000; ICC/IF: 1:100-1000; FCM: 1:100-1000 |
Antibody Type | Polyclonal |
Host Species | Goat |
Construction | Polyclonal Antibody |
Purification | Affinity purified, nonspecific adsorbed |
Appearance | Liquid |
Formulation | 10 mM TBS (pH=7.4) with 1% BSA, 0.03% Proclin300 and 50% glycerol. |
Research Background | Immunoglobulin G (IgG) is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants. |
Immunogen | Native Rabbit IgG |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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