Autophagyinducer 7 (Compound SSA) serves as an inducer of autophagy (Autophagy) and apoptosis (Apoptosis). It stimulates autophagy by inhibiting Akt/mTOR signaling and the expression of downstream proteins. Additionally, Autophagyinducer 7 suppresses DNA synthesis and causes G0-G1 cell cycle arrest, ultimately inhibiting the growth of tumor cells.

Autophagy inducer 7 inhibits the growth of human lung adenocarcinoma cell lines with IC50 values of 1.95 μM (HOP-62), 4.72 μM (A549), and 3.05 μM (H1299) at 72 hours. At concentrations of 0-10 μM over 24 hours, it induces apoptosis in HOP-62, A549, and H1299 cells, but less effectively than Sulindac sulfide and Staurosporine. Additionally, Autophagy inducer 7 at 0-7.5 μM for 24 hours suppresses DNA synthesis in the same cell lines and raises the percentage of cells in the G0-G1 phase of the cell cycle. It also induces autophagy and enhances autophagic flux in A549 cells by inhibiting Akt/mTOR/p70S6k signaling.