Anti-XTP4 Polyclonal Antibody is a Rabbit antibody targeting XTP4. Anti-XTP4 Polyclonal Antibody can be used in FCM.

IgG

Mouse (predicted:Human,Rat,Dog,Pig,Cow,Horse,Rabbit,Sheep)

1. Blank control: Mouse kidney.
Primary Antibody (green line): Rabbit Anti-XTP4 antibody (TMAB-14225)
Dilution: 2 μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-AF488
Dilution: 1 μg /test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

FCM: 2μg/Test

Polyclonal

KLH conjugated synthetic peptide: human XTP4

Human

MIEN1

Migration and invasion enhancer 1

Increases cell migration by inducing filopodia formation at the leading edge of migrating cells. Plays a role in regulation of apoptosis, possibly through control of CASP3. May be involved in a redox-related process.