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Anti-XBP1 Antibody (8M292)
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Catalog No. TMAC-04296
Alias XBP2, XBP-1, XBP1, XBP 1, X-box-binding protein 1, luminal form, X-box-binding protein 1, cytoplasmic form, X-box-binding protein 1 in isoform 2, X box-binding protein-1, X box binding protein 2, TREB5, Tax responsive element binding protein 5
Anti-XBP1 Antibody (8M292) is a Rabbit antibody targeting XBP1. Anti-XBP1 Antibody (8M292) can be used in WB,IHC,ICC/IF,FCM.
Anti-XBP1 Antibody (8M292)
Anti-XBP1 Antibody (8M292)
😃Good
Catalog No. TMAC-04296Alias XBP2, XBP-1, XBP1, XBP 1, X-box-binding protein 1, luminal form, X-box-binding protein 1, cytoplasmic form, X-box-binding protein 1 in isoform 2, X box-binding protein-1, X box binding protein 2, TREB5, Tax responsive element binding protein 5
Anti-XBP1 Antibody (8M292) is a Rabbit antibody targeting XBP1. Anti-XBP1 Antibody (8M292) can be used in WB,IHC,ICC/IF,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $296 | 7-10 days | |
| 100 μL | $496 | 7-10 days |
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Chemical Properties
| Description | Anti-XBP1 Antibody (8M292) is a Rabbit antibody targeting XBP1. Anti-XBP1 Antibody (8M292) can be used in WB,IHC,ICC/IF,FCM. |
| Synonyms | XBP2, XBP-1, XBP1, XBP 1, X-box-binding protein 1, luminal form, X-box-binding protein 1, cytoplasmic form, X-box-binding protein 1 in isoform 2, X box-binding protein-1, X box binding protein 2, TREB5, Tax responsive element binding protein 5 |
| Ig Type | IgG |
| Reactivity | Human |
| Verified Activity | 1. Western blot analysis of XBP1 on different cell lysates using anti-XBP1 antibody at 1/1,000 dilution. Positive control: Lane 1: HepG2, Lane 2: 293T. 2. Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-XBP1 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-XBP1 antibody. Counter stained with hematoxylin. 4. ICC staining XBP1 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 5. ICC staining XBP1 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. ICC staining XBP1 in SW480 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. Flow cytometric analysis of 293T cells with XBP1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
| Application | |
| Recommended Dose | WB: 1:500-1000; IHC: 1:50-200; ICC/IF: 1:100-500; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | The X-box binding protein-1 (XBP-1 or hXBP-1), also designated tax-responsive element-binding protein 5 (TREB5) in mouse and human, or hepatocarcinogenesis-related transcription factor (HTF) in rat, belongs to the basic region/leucine zipper (bZIP) family of transcription factors. XBP-1 was first characterized as a protein that binds to the HLA-DRα promoter in B cells. XBP-1 recognizes the cAMP responsive element (CRE) in enhancers of human T cell leukemia virus and major histocompatibility complex class II genes and activates transcription of these genes. It is expressed at high levels in developing bone and its levels are modulated during osteoblast development, suggesting a role in regulation of expression of osteoblast-specific genes. In addition to binding to CRE sequences, XBP-1 has been shown to bind to TPA response elements (TREs). |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 40 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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