Anti-Vitamin D Receptor/VDR Polyclonal Antibody is a Rabbit antibody targeting Vitamin D Receptor/VDR. Anti-Vitamin D Receptor/VDR Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF,FCM.

IgG

Human,Mouse (predicted:Rat,Dog,Pig,Cow,Horse,Rabbit,Sheep)

1. Blank control: U937. Primary Antibody (green line): Rabbit Anti-Vitamin D Receptor antibody (TMAB-01970)
Dilution: 1 μg/Test;
Secondary Antibody: Goat anti-rabbit IgG-FITC
Dilution: 0.5 μg/Test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
2. Tissue/cell: mouse embryo tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-Vitamin D Receptor Polyclonal Antibody, Unconjugated (TMAB-01970) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.

IHC-P,IHC-Fr,IF,FCM

IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1ug/Test

Polyclonal

KLH conjugated synthetic peptide: human Vitamin D Receptor

Human

Vitamin D Receptor,Zinc Finger,Vitamin D Receptor,Nuclear hormone receptors