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Anti-villin1 Antibody (7N900)
😃Good
Catalog No. TMAC-04254
Alias Villin-1, VILI_HUMAN, VIL1, VIL, OTTHUMP00000164145, D2S1471
Anti-villin1 Antibody (7N900) is a Rabbit antibody targeting villin1. Anti-villin1 Antibody (7N900) can be used in WB,ICC,IHC,FCM.
Anti-villin1 Antibody (7N900)
Anti-villin1 Antibody (7N900)
😃Good
Catalog No. TMAC-04254Alias Villin-1, VILI_HUMAN, VIL1, VIL, OTTHUMP00000164145, D2S1471
Anti-villin1 Antibody (7N900) is a Rabbit antibody targeting villin1. Anti-villin1 Antibody (7N900) can be used in WB,ICC,IHC,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $296 | 7-10 days | |
| 100 μL | $496 | 7-10 days |
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Antigen Details
Chemical Properties
| Description | Anti-villin1 Antibody (7N900) is a Rabbit antibody targeting villin1. Anti-villin1 Antibody (7N900) can be used in WB,ICC,IHC,FCM. |
| Synonyms | Villin-1, VILI_HUMAN, VIL1, VIL, OTTHUMP00000164145, D2S1471 |
| Ig Type | IgG |
| Clone | 7N900 |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Western blot analysis of Villin1 on different tissue lysates using anti-Villin1 antibody at 1/500 dilution. Positive control: Lane 1: Mouse colon, Lane 2: Human small intestine, Lane 3: Human colonm Lane 4: Rat kidney. 2. Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Villin1 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Villin1 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Villin1 antibody. Counter stained with hematoxylin. 5. ICC staining Villin1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. ICC staining Villin1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. ICC staining Villin1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 8. Flow cytometric analysis of Hela cells with Villin1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC: 1:50-200; ICC: 1:500-2000; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | Caldesmon, Filamin 1, Nebulin and Villin are differentially expressed and regulated Actin binding proteins. Both muscular (CDh) and non-muscular (CDl) forms of Caldesmon have been identified and each has been shown to bind to Actin as well as to calmodulin and myosin. CDh is expressed predominantly on thin filaments in smooth muscle, whereas CDl is widely expressed in non-muscle tissues and cells. Filamin 1, which is ubiquitously expressed and exists as a homodimer, functions to crosslink Actin to filaments. Nebulin is a large filamentous protein specific to muscle tissue that may function as a ruler for filament length. Several isoforms of Nebulin are produced by alternative exon usage. Villin is Ca2+-regulated and is the major structural component of the brush border of absorptive cells. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 92/46 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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