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Anti-USP22 Antibody (1F346)
😃Good
Catalog No. TMAC-04205
Alias USP3L, USP 22, Ubiquitin-specific-processing protease 22, Ubiquitin thioesterase 22, Ubiquitin carboxyl-terminal hydrolase 22, KIAA1063, EC 3.4.19.12, Deubiquitinating enzyme 22
Anti-USP22 Antibody (1F346) is a Rabbit antibody targeting USP22. Anti-USP22 Antibody (1F346) can be used in FCM,ICC/IF,IP,WB.
Anti-USP22 Antibody (1F346)
Anti-USP22 Antibody (1F346)
😃Good
Catalog No. TMAC-04205Alias USP3L, USP 22, Ubiquitin-specific-processing protease 22, Ubiquitin thioesterase 22, Ubiquitin carboxyl-terminal hydrolase 22, KIAA1063, EC 3.4.19.12, Deubiquitinating enzyme 22
Anti-USP22 Antibody (1F346) is a Rabbit antibody targeting USP22. Anti-USP22 Antibody (1F346) can be used in FCM,ICC/IF,IP,WB.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $296 | 7-10 days | |
| 100 μL | $498 | 7-10 days |
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Chemical Properties
| Description | Anti-USP22 Antibody (1F346) is a Rabbit antibody targeting USP22. Anti-USP22 Antibody (1F346) can be used in FCM,ICC/IF,IP,WB. |
| Synonyms | USP3L, USP 22, Ubiquitin-specific-processing protease 22, Ubiquitin thioesterase 22, Ubiquitin carboxyl-terminal hydrolase 22, KIAA1063, EC 3.4.19.12, Deubiquitinating enzyme 22 |
| Ig Type | IgG |
| Clone | 1F346 |
| Reactivity | Human |
| Verified Activity | 1. Western blot analysis of USP22 on Hela (1) and HepG2 (2) cell lysate using anti-USP22 antibody at 1/500 dilution. 2. ICC staining USP22 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 3. ICC staining USP22 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 4. ICC staining USP22 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 5. Flow cytometric analysis of Hela cells with USP22 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
| Application | |
| Recommended Dose | WB: 1:1000-5000; ICC/IF: 1:50-200; IP: 1:10-50; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | The ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates. Through the use of a wide range of enzymes that can add or remove ubiquitin, the Ub pathway controls many intracellular processes such as signal transduction, transcriptional activation and cell cycle progression. USP22 (ubiquitin specific peptidase 22), also known as USP3L, is a 525 amino acid protein that contains one UBP-type zinc finger and functions to catalyze the conversion of a ubiquitin C-terminal thioester to free ubiquitin and thiol, a reaction that may influence several cellular processes. Via its catalytic activity, USP22 is thought to play an important role in cell cycle progression and may also serve as a cancer stem cell marker. The gene encoding USP22 maps to human chromosome 17, which comprises over 2.5% of the human genome and encodes over 1,200 genes. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 60 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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