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Anti-Transglutaminase 2 Polyclonal Antibody 2
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Catalog No. TMAB-13732
Anti-Transglutaminase 2 Polyclonal Antibody 2 is a Rabbit antibody targeting Transglutaminase 2. Anti-Transglutaminase 2 Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,IF.
Anti-Transglutaminase 2 Polyclonal Antibody 2
Anti-Transglutaminase 2 Polyclonal Antibody 2
Copy Product Info😃Good
Catalog No. TMAB-13732
Anti-Transglutaminase 2 Polyclonal Antibody 2 is a Rabbit antibody targeting Transglutaminase 2. Anti-Transglutaminase 2 Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $220 | 7-10 days | 7-10 days | |
| 100 μL | $373 | 7-10 days | 7-10 days | |
| 200 μL | $528 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Antigen Details
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| Description | Anti-Transglutaminase 2 Polyclonal Antibody 2 is a Rabbit antibody targeting Transglutaminase 2. Anti-Transglutaminase 2 Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Human,Mouse (predicted:Rat,Dog,Pig,Cow,Horse,Rabbit,Sheep) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (TGM2) Polyclonal Antibody, Unconjugated (TMAB-13732) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded (human Pancreatic cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (TGM2) Polyclonal Antibody, Unconjugated (TMAB-13732) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 3. Sample: Uterus (Mouse) Lysate at 40 μg Primary: Anti-Transglutaminase 2 (TMAB-13732) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 77 kD Observed band size: 74 kD |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | Transglutaminases are enzymes that catalyze the crosslinking of proteins by epsilon-gamma glutamyl lysine isopeptide bonds. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium-dependent. The protein encoded by this gene acts as a monomer, is induced by retinoic acid, and appears to be involved in apoptosis. Finally, the encoded protein is the autoantigen implicated in celiac disease. Two transcript variants encoding different isoforms have been found for this gene. |
| Immunogen | KLH conjugated synthetic peptide: human Transglutaminase 2 |
| Antigen Species | Human |
| Gene Name | TGM2 |
| Gene ID | |
| Protein Name | Protein-glutamine gamma-glutamyltransferase 2 |
| Uniprot ID | |
| Function | Catalyzes the cross-linking of proteins and the conjugation of polyamines to proteins. |
| Molecular Weight | Theoretical: 77 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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