Anti-Transferrin Receptor/TFRC Antibody (4F227) is a Rabbit antibody targeting Transferrin Receptor/TFRC. Anti-Transferrin Receptor/TFRC Antibody (4F227) can be used in FCM,ICC/IF,WB.

IgG

4F227

Human,Rat (predicted:Mouse)

1. 25 μg total protein per Lane of various lysates probed with TFRC monoclonal antibody, unconjugated (TMAB-01889) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min.
2. The Jar (H) cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20°C,the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at room temperature). Primary Antibody (green): Rabbit Anti-TFRC antibody (TMAB-01889): 1 μg/10^6 cells; Secondary Antibody (white blue): Goat anti-Rabbit IgG-FITC: 1 μg/test. Isotype Control (orange): Rabbit IgG. Blank control (black): PBS.
3. 4% Paraformaldehyde-fixed Hela (H) cell; Triton X-100 at RT for 20 min; Antibody incubation with (TFRC) monoclonal Antibody, unconjugated (TMAB-01889) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green ) at 37°C for 90 min, DAPI (blue) was used to stain the cell nucleus. PBS instead of the primary antibody was used as the blank control.

WB: 1:500-2000; ICC/IF: 1:50-200; FCM: 1μg/Test

Monoclonal

A synthesized peptide: human Transferrin receptor

Human

TFRC

Transferrin receptor protein 1

Response to hypoxia,Serum Proteins,Non-lineage,Hypoxia,B Lymphocytic Lineage,T Lymphocytic Lineage,Erythrocytic Lineage,Surface Molecules

Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system. A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferring for an overlapping C-terminal binding site.